Botrytis cinerea, the main pathogen of strawberry, has the ability to remain quiescent in unripe tissue and develop disease symptoms in ripe fruit. As strawberry ripening is characterized by an increase of aroma compounds, the role of volatile emission in the development of infection was investigated. Thirty-five strawberry volatile organic compounds (VOCs) were tested on B. cinerea in vitro and volatile emission was analysed in strawberry harvested at four ripening stages by headspace solid-phase microextraction/gas chromatography–mass spectrometry and proton transfer reaction– time of flight–mass spectrometry. The coupling of such data sets made it possible to conclude that key strawberry aroma compounds stimulate B. cinerea conidial germination and some typical wound-volatiles stimulate pathogen conidial germination or mycelial growth. This study is the first report of fungal stimulation by some VOCs naturally occurring in strawberry: the esters ethyl butanoate, cis-3-hexenyl acetate, trans-2-hexenyl acetate, methyl butanoate and hexyl butanoate, the furanones furaneol and mesifurane, and the alcohol trans-2-hexenol. The results of this work provide advances in understanding the functional role of fruit VOCs and suggest, for the first time, that fruit VOCs may influence the development of B. cinerea from the latent phase and that they could favour the invasive growth of B. cinerea after wounding. In particular, ethyl butanoate and furaneol could signal strawberry ripening, and the green leaf volatiles trans-2-hexenol, trans-2-hexenyl acetate and cis-3-hexenyl acetate could signal the presence of damaged tissues that are easier sites for penetration by B. cinerea

Neri, F.; Cappellin, L.; Spadoni, A.; Cameldi, I.; Algarra Alarcon, A.; Aprea, E.; Romano, A.; Gasperi, F.; Biasioli, F. (2015). Role of strawberry volatile organic compounds in the development of Botrytis cinerea infection. PLANT PATHOLOGY, 64 (3): 709-717. doi: 10.1111/ppa.12287 handle: http://hdl.handle.net/10449/25035

Role of strawberry volatile organic compounds in the development of Botrytis cinerea infection

Cappellin, Luca;Algarra Alarcon, Alberto;Aprea, Eugenio;Romano, Andrea;Gasperi, Flavia;Biasioli, Franco
2015-01-01

Abstract

Botrytis cinerea, the main pathogen of strawberry, has the ability to remain quiescent in unripe tissue and develop disease symptoms in ripe fruit. As strawberry ripening is characterized by an increase of aroma compounds, the role of volatile emission in the development of infection was investigated. Thirty-five strawberry volatile organic compounds (VOCs) were tested on B. cinerea in vitro and volatile emission was analysed in strawberry harvested at four ripening stages by headspace solid-phase microextraction/gas chromatography–mass spectrometry and proton transfer reaction– time of flight–mass spectrometry. The coupling of such data sets made it possible to conclude that key strawberry aroma compounds stimulate B. cinerea conidial germination and some typical wound-volatiles stimulate pathogen conidial germination or mycelial growth. This study is the first report of fungal stimulation by some VOCs naturally occurring in strawberry: the esters ethyl butanoate, cis-3-hexenyl acetate, trans-2-hexenyl acetate, methyl butanoate and hexyl butanoate, the furanones furaneol and mesifurane, and the alcohol trans-2-hexenol. The results of this work provide advances in understanding the functional role of fruit VOCs and suggest, for the first time, that fruit VOCs may influence the development of B. cinerea from the latent phase and that they could favour the invasive growth of B. cinerea after wounding. In particular, ethyl butanoate and furaneol could signal strawberry ripening, and the green leaf volatiles trans-2-hexenol, trans-2-hexenyl acetate and cis-3-hexenyl acetate could signal the presence of damaged tissues that are easier sites for penetration by B. cinerea
Botrytis cinerea
Latent infection
Proton transfer reaction-time-of-flight–mass spectrometry
Solid phase microextraction/ gas chromatography–mass spectrometry
Strawberry
Volatile organic compounds
Settore AGR/12 - PATOLOGIA VEGETALE
2015
Neri, F.; Cappellin, L.; Spadoni, A.; Cameldi, I.; Algarra Alarcon, A.; Aprea, E.; Romano, A.; Gasperi, F.; Biasioli, F. (2015). Role of strawberry volatile organic compounds in the development of Botrytis cinerea infection. PLANT PATHOLOGY, 64 (3): 709-717. doi: 10.1111/ppa.12287 handle: http://hdl.handle.net/10449/25035
File in questo prodotto:
File Dimensione Formato  
2015 PP Neri et al.pdf

solo utenti autorizzati

Licenza: Tutti i diritti riservati (All rights reserved)
Dimensione 514.8 kB
Formato Adobe PDF
514.8 kB Adobe PDF   Visualizza/Apri   Richiedi una copia

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10449/25035
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 43
  • ???jsp.display-item.citation.isi??? 40
social impact