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Phytochelatin synthase de-regulation in Marchantia polymorpha indicates cadmium detoxification as its primary ancestral function in land plants and provides a novel visual bioindicator for detection of this metal

Bryophytes are a large group of plants commonly used as bioindicators of metal and metalloid pollution. However, very little is known about the bryophyte genes responsible for metal(loid) detoxification, a knowledge that could provide novel tools for environmental monitoring applications. By genetic transformation and genome editing, in this study we obtained lines of the model bryophyte Marchantia polymorpha with de-regulated activity of the phytochelatin synthase (MpPCS) enzyme, responsibl...

Electrophysiologically determined spectral responses in Lobesia botrana (Lepidoptera: Tortricidae)

Electrophysiological methods were used to test the visual sensitivity of European grapevine moth, Lobesia botrana (Lepidoptera: Tortricidae) to wavelengths ranging from 300 to 700 nm. For male and females tested, a main, peak response occurred in the 460-540 nm range (blue-green wavelengths) with females having a generally lower response to wavelengths in that range. A second smaller peak was observed for both sexes at the 340-420 nm range. A general linear model indicated that males, virgin ...

Characterization of botanical origin of Italian honey by carbohydrate composition and Volatile Organic Compounds (VOCs)

Honey is a natural sweetener constituted by numerous macro- and micronutrients. Carbohydrates are the most representative, with glucose and fructose being the most abundant. Minor honey components like volatile organic compounds (VOCs), minerals, vitamins, amino acids are able to confer honey-specific properties and are useful to characterize and differentiate between honey varieties according to the botanical origin. The present work describes the chemical characterization of honeys of diffe...

Interlaboratory performance of a Real-Time PCR method for detection of Ceratocystis platani, the agent of canker stain of Platanus spp

Ceratocystis platani (CP), an ascomycetous fungus, is the agent of canker stain, a lethal vascular disease of Platanus species. Ceratocystis platani has been listed as a quarantine pest (EPPO A2 list) due to extensive damage caused in Southern Europe and the Mediterranean region. As traditional diagnostic assays are ineffective, a Real-Time PCR detection method based on EvaGreen, SYBR Green, and Taqman assays was previously developed, validated in-house, and included in the official EPPO stan...

Burro di malga: una specialità di oggi con profonde radici nel passato

Una nicchia nella nicchia dove l'elevata qualità è intrinsecamente legata alla tradizione e alla ricchezza in biodiversità microbica che porta a uno sviluppo aromatico molto interessante

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