Compelling evidence of the health benefits of phenolic compounds and their impact on food quality have stimulated the development of analytical methods for the identification and quantification of these compounds in different matrices in recent years. We have developed a targeted metabolomics method for the quantification of 140 phenolics,such as benzoates, phenylpropanoids, coumarins, stilbenes, dihydrochalcones and flavonoids in fruit and tea extracts and wine using UPLC-MS/MS. Chromatography was optimised to achieve separation of the compounds over a period of 15 minutes and MRM transitions were selected for accurate quantification. The effectiveness of the method was validated by studying the detection and quantification limits, the linearity ranges of instrumental response and the repeatability of the analysis. The method was validated for the analysis of apples, berries, green tea and red wine and represents a valuable tool for food quality evaluation and breeding studies.

Vrhovsek, U.; Masuero, D.; Gasperotti, M.; Franceschi, P.; Caputi, L.; Viola, R.; Mattivi, F. (2012). A versatile targeted metabolomics method for rapid quantification of multiple classes of phenolics in fruit and beverages. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 60 (36): 8831-8840. doi: 10.1021/jf2051569 handle: http://hdl.handle.net/10449/20912

A versatile targeted metabolomics method for rapid quantification of multiple classes of phenolics in fruit and beverages

Vrhovsek, Urska;Masuero, Domenico;Gasperotti, Mattia;Franceschi, Pietro;Caputi, Lorenzo;Viola, Roberto;Mattivi, Fulvio
2012-01-01

Abstract

Compelling evidence of the health benefits of phenolic compounds and their impact on food quality have stimulated the development of analytical methods for the identification and quantification of these compounds in different matrices in recent years. We have developed a targeted metabolomics method for the quantification of 140 phenolics,such as benzoates, phenylpropanoids, coumarins, stilbenes, dihydrochalcones and flavonoids in fruit and tea extracts and wine using UPLC-MS/MS. Chromatography was optimised to achieve separation of the compounds over a period of 15 minutes and MRM transitions were selected for accurate quantification. The effectiveness of the method was validated by studying the detection and quantification limits, the linearity ranges of instrumental response and the repeatability of the analysis. The method was validated for the analysis of apples, berries, green tea and red wine and represents a valuable tool for food quality evaluation and breeding studies.
Metabolite profiling
Polyphenols
Food analysis
UPLC-MS/MS
Mass spectrometry
Settore CHIM/10 - CHIMICA DEGLI ALIMENTI
2012
Vrhovsek, U.; Masuero, D.; Gasperotti, M.; Franceschi, P.; Caputi, L.; Viola, R.; Mattivi, F. (2012). A versatile targeted metabolomics method for rapid quantification of multiple classes of phenolics in fruit and beverages. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 60 (36): 8831-8840. doi: 10.1021/jf2051569 handle: http://hdl.handle.net/10449/20912
File in questo prodotto:
File Dimensione Formato  
2012 JAFC Vrhovsek et al.pdf

solo utenti autorizzati

Licenza: Tutti i diritti riservati (All rights reserved)
Dimensione 330.59 kB
Formato Adobe PDF
330.59 kB Adobe PDF   Visualizza/Apri   Richiedi una copia

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10449/20912
Citazioni
  • ???jsp.display-item.citation.pmc??? 84
  • Scopus 258
  • ???jsp.display-item.citation.isi??? 250
social impact