The aim of this paper is to provide a detailed characterisation of grape lipidome. To achieve this objective, it starts by describing a pipeline implemented in R software to allow the semi-automatic annotation of the detected lipid species. It also provides an extensive description of the different properties of each molecule (such as retention time dependencies, mass accuracy, adduct formation and fragmentation patterns), which allowed the annotations to be made more accurately. Most annotated lipids in the grape samples were (lyso)glycerophospholipids and glycerolipids, although a few free fatty acids, hydroxyceramides and sitosterol esters were also observed. The proposed pipeline also allowed the identification of a series of methylated glycerophosphates never previously observed in grapes. The current results highlight the importance of expanding chemical analyses beyond the classical lipid categories.

Garcia Aloy, M.; Masuero, D.; Chitarrini, G.; Skrab, D.; Sivilotti, P.; Guella, G.; Vrhovsek, U.; Franceschi, P. (2023). Untargeted lipidomic profiling of grapes highlights the importance of modified lipid species beyond the traditional compound classes. FOOD CHEMISTRY, 410: 135360. doi: 10.1016/j.foodchem.2022.135360 handle: https://hdl.handle.net/10449/78015

Untargeted lipidomic profiling of grapes highlights the importance of modified lipid species beyond the traditional compound classes

Garcia Aloy, M.
Primo
;
Masuero, D.;Chitarrini, G.;Skrab, D.;Vrhovsek, U.;Franceschi, P.
Ultimo
2023-01-01

Abstract

The aim of this paper is to provide a detailed characterisation of grape lipidome. To achieve this objective, it starts by describing a pipeline implemented in R software to allow the semi-automatic annotation of the detected lipid species. It also provides an extensive description of the different properties of each molecule (such as retention time dependencies, mass accuracy, adduct formation and fragmentation patterns), which allowed the annotations to be made more accurately. Most annotated lipids in the grape samples were (lyso)glycerophospholipids and glycerolipids, although a few free fatty acids, hydroxyceramides and sitosterol esters were also observed. The proposed pipeline also allowed the identification of a series of methylated glycerophosphates never previously observed in grapes. The current results highlight the importance of expanding chemical analyses beyond the classical lipid categories.
Lipidomics
Annotation
Grape
Mass spectrometry
Ripening
Plant tissues
Settore CHIM/01 - CHIMICA ANALITICA
Garcia Aloy, M.; Masuero, D.; Chitarrini, G.; Skrab, D.; Sivilotti, P.; Guella, G.; Vrhovsek, U.; Franceschi, P. (2023). Untargeted lipidomic profiling of grapes highlights the importance of modified lipid species beyond the traditional compound classes. FOOD CHEMISTRY, 410: 135360. doi: 10.1016/j.foodchem.2022.135360 handle: https://hdl.handle.net/10449/78015
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10449/78015
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