Alpine pastures have not yet been extensively studied with regard to the presence of alkaloids in herbaceous plants. In this work the alkaloid profiles were characterised from a selection of 62 herbs collected from alpine pastures in north-eastern Italy. High-performance liquid chromatography coupled to a hybrid quadrupole-orbitrap mass spectrometer was used to evaluate the presence of 41 different alkaloids and quantify them using a targeted approach. Provisionally, 118 alkaloids were identified, including both free and glycosylated forms, making use of a homemade database and a suspect screening approach. rucifoline, gramine, heliotrine, lycopsamine, seneciphylline, and veratramine were quantified with concentrations ranging from 6 to about 100 µg kg-1 in 6 plants. Herbaceous plants belonging to the most well-represented plant families (Poaceae, 9 species; Asteraceae, 7; Lamiaceae, 6) showed distinct and characteristic alkaloid profiles and were correctly reclassified with an average accuracy of 85% (Partial Least Squares - Discriminant Analysis).

Nardin, T.; Larcher, R.; Barnaba, C.; Bertoldi, D.; Pasut, D.; Romanzin, A.; Piasentier, E. (2023-03-12). Alkaloid profiling of Italian alpine herbs using high resolution mass spectrometry (Orbitrap-MS). NATURAL PRODUCT RESEARCH, 37 (15): 2583-2590. doi: 10.1080/14786419.2022.2050908 handle: https://hdl.handle.net/10449/77418

Alkaloid profiling of Italian alpine herbs using high resolution mass spectrometry (Orbitrap-MS)

Nardin, Tiziana
Primo
;
Larcher, Roberto
;
Barnaba, Chiara;Bertoldi, Daniela;
2023-03-12

Abstract

Alpine pastures have not yet been extensively studied with regard to the presence of alkaloids in herbaceous plants. In this work the alkaloid profiles were characterised from a selection of 62 herbs collected from alpine pastures in north-eastern Italy. High-performance liquid chromatography coupled to a hybrid quadrupole-orbitrap mass spectrometer was used to evaluate the presence of 41 different alkaloids and quantify them using a targeted approach. Provisionally, 118 alkaloids were identified, including both free and glycosylated forms, making use of a homemade database and a suspect screening approach. rucifoline, gramine, heliotrine, lycopsamine, seneciphylline, and veratramine were quantified with concentrations ranging from 6 to about 100 µg kg-1 in 6 plants. Herbaceous plants belonging to the most well-represented plant families (Poaceae, 9 species; Asteraceae, 7; Lamiaceae, 6) showed distinct and characteristic alkaloid profiles and were correctly reclassified with an average accuracy of 85% (Partial Least Squares - Discriminant Analysis).
HQOMS
Alkaloids
Alpine herbs
Settore CHIM/10 - CHIMICA DEGLI ALIMENTI
12-mar-2023
Nardin, T.; Larcher, R.; Barnaba, C.; Bertoldi, D.; Pasut, D.; Romanzin, A.; Piasentier, E. (2023-03-12). Alkaloid profiling of Italian alpine herbs using high resolution mass spectrometry (Orbitrap-MS). NATURAL PRODUCT RESEARCH, 37 (15): 2583-2590. doi: 10.1080/14786419.2022.2050908 handle: https://hdl.handle.net/10449/77418
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