To elucidate the physiology underlying the development of superficial scald in pears, susceptible "Blanquilla" fruit was treated with different compounds that either promoted (ethylene) or repressed (1-methylcyclopropene and lovastatin) the incidence of this disorder after 4 months of cold storage. Our data show that scald was negligible for the fruit treated with 1-methylcyclopropene or lovastatin, but highly manifested in untreated (78% incidence) or ethylene-treated fruit (97% incidence). The comparison between the fruit metabolomic profile and transcriptome evidenced a distinct reprogramming associated with each treatment. In all treated samples, cold storage led to an activation of a cold-acclimation-resistance mechanism, including the biosynthesis of very-long-chain fatty acids, which was especially evident in 1-methylcyclopropane-treated fruit. Among the treatments applied, only 1-methylcyclopropene inhibited ethylene production, hence supporting the involvement of this hormone in the development of scald. However, a common repression effect on the PPO gene combined with higher sorbitol content was found for both lovastatin and 1-methylcyclopropene-treated samples, suggesting also a non-ethylene-mediated process preventing the development of this disorder. The results presented in this work represent a step forward to better understand the physiological mechanisms governing the etiology of superficial scald in pears.

Giné-Bordonaba, J.; Busatto, N.; Larrigaudière, C.; Lindo-García, V.; Echeverria, G.; Vrhovsek, U.; Farneti, B.; Biasioli, F.; De Quattro, C.; Rossato, M.; Delledonne, M.; Costa, F. (2020). Investigation of the Transcriptomic and Metabolic Changes Associated With Superficial Scald Physiology Impaired by Lovastatin and 1-methylcyclopropene in Pear Fruit (Cv. "Blanquilla"). HORTICULTURE RESEARCH, 7: 49. doi: 10.1038/s41438-020-0272-x handle: http://hdl.handle.net/10449/57797

Investigation of the Transcriptomic and Metabolic Changes Associated With Superficial Scald Physiology Impaired by Lovastatin and 1-methylcyclopropene in Pear Fruit (Cv. "Blanquilla")

Busatto, N.;Vrhovsek, U.;Farneti, B.;Biasioli, F.;Costa, F.
Ultimo
2020-01-01

Abstract

To elucidate the physiology underlying the development of superficial scald in pears, susceptible "Blanquilla" fruit was treated with different compounds that either promoted (ethylene) or repressed (1-methylcyclopropene and lovastatin) the incidence of this disorder after 4 months of cold storage. Our data show that scald was negligible for the fruit treated with 1-methylcyclopropene or lovastatin, but highly manifested in untreated (78% incidence) or ethylene-treated fruit (97% incidence). The comparison between the fruit metabolomic profile and transcriptome evidenced a distinct reprogramming associated with each treatment. In all treated samples, cold storage led to an activation of a cold-acclimation-resistance mechanism, including the biosynthesis of very-long-chain fatty acids, which was especially evident in 1-methylcyclopropane-treated fruit. Among the treatments applied, only 1-methylcyclopropene inhibited ethylene production, hence supporting the involvement of this hormone in the development of scald. However, a common repression effect on the PPO gene combined with higher sorbitol content was found for both lovastatin and 1-methylcyclopropene-treated samples, suggesting also a non-ethylene-mediated process preventing the development of this disorder. The results presented in this work represent a step forward to better understand the physiological mechanisms governing the etiology of superficial scald in pears.
Abiotic
Plant hormones
Settore AGR/07 - GENETICA AGRARIA
2020
Giné-Bordonaba, J.; Busatto, N.; Larrigaudière, C.; Lindo-García, V.; Echeverria, G.; Vrhovsek, U.; Farneti, B.; Biasioli, F.; De Quattro, C.; Rossato, M.; Delledonne, M.; Costa, F. (2020). Investigation of the Transcriptomic and Metabolic Changes Associated With Superficial Scald Physiology Impaired by Lovastatin and 1-methylcyclopropene in Pear Fruit (Cv. "Blanquilla"). HORTICULTURE RESEARCH, 7: 49. doi: 10.1038/s41438-020-0272-x handle: http://hdl.handle.net/10449/57797
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