Herbal infusions are consumed worldwide thanks to their “natural” beneficial effects, also due to the presence of alkaloids, although these compounds can have poisonous effects. A method combining online solid-phase purification with high resolution mass spectrometry was used to define the alkaloid profiles of 117 herbs and 7 commercial blends. 41 alkaloids were quantified in reference to analytical standards, while the presence of a further 116 was confirmed on the basis of accurate mass, retention time and fragmentation profile. The targeted study showed that 52% of herbs and 42% of commercial blends contained at least one alkaloid, and pyrrolizidines were the most commonly present (26% of samples), with concentrations generally ranging from the quantification limit to roughly 100 μg kg-1. Moreover, a homemade infusion was studied, finding on average 45% and 6% lower extraction for pyrrolizidine and steroidal alkaloids, respectively. Nevertheless, the migration of pyrrolizidines was confirmed. The study confirmed the frequent presence, natural or accidental, of alkaloids in commercial infusion herbs, highlighting the urgent need for routine and accurate controls

Nardin, T.; Piasentier, E.; Barnaba, C.; Larcher, R. (2018). Alkaloid profiling of herbal drugs using high resolution mass spectrometry. DRUG TESTING AND ANALYSIS, 10 (3): 423-448. doi: 10.1002/dta.2252 handle: http://hdl.handle.net/10449/43102

Alkaloid profiling of herbal drugs using high resolution mass spectrometry

Nardin, Tiziana
Primo
;
Barnaba, Chiara;Larcher, Roberto
Ultimo
2018

Abstract

Herbal infusions are consumed worldwide thanks to their “natural” beneficial effects, also due to the presence of alkaloids, although these compounds can have poisonous effects. A method combining online solid-phase purification with high resolution mass spectrometry was used to define the alkaloid profiles of 117 herbs and 7 commercial blends. 41 alkaloids were quantified in reference to analytical standards, while the presence of a further 116 was confirmed on the basis of accurate mass, retention time and fragmentation profile. The targeted study showed that 52% of herbs and 42% of commercial blends contained at least one alkaloid, and pyrrolizidines were the most commonly present (26% of samples), with concentrations generally ranging from the quantification limit to roughly 100 μg kg-1. Moreover, a homemade infusion was studied, finding on average 45% and 6% lower extraction for pyrrolizidine and steroidal alkaloids, respectively. Nevertheless, the migration of pyrrolizidines was confirmed. The study confirmed the frequent presence, natural or accidental, of alkaloids in commercial infusion herbs, highlighting the urgent need for routine and accurate controls
Alkaloids
Herbal infusion
Liquid chromatography
OrbitrapTM
On-line solid-phase extraction
Settore CHIM/10 - CHIMICA DEGLI ALIMENTI
Nardin, T.; Piasentier, E.; Barnaba, C.; Larcher, R. (2018). Alkaloid profiling of herbal drugs using high resolution mass spectrometry. DRUG TESTING AND ANALYSIS, 10 (3): 423-448. doi: 10.1002/dta.2252 handle: http://hdl.handle.net/10449/43102
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