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ItalianoThe main aim of this work was to develop an untargeted normal phase LC–MS method, starting from a targeted method already validated for the analysis of 135 polar metabolites. Since the LC instrument and column were the same, most of the chromatographic conditions remained identical, while the adaptations focused on maintaining the ionic strength of the eluents constant. The sample preparation was simplified and the effectiveness of LC–MS for long batches was evaluated, in order to record the maximum number of metabolites with good chromatographic resolution and the best MS stability and accuracy. The method was applied to study the influence of storage conditions on wine composition. Slightly sub-optimum storage conditions had a major impact on the polar metabolite fingerprint of the red wines analysed and the markers revealed included phenolics, vitamins and metabolites indentified in wine for the first time (4-amino-heptanedioic acid and its ethyl ester)
| Citation: | Arapitsas, P.; Della Corte, A.; Gika, H.; Narduzzi, L.; Mattivi, F.; Theodoridis, G. (2016). Studying the effect of storage conditions on the metabolite content of red wine using HILIC LC–MS based metabolomics. FOOD CHEMISTRY, 497 (Part B): 1331-1340. doi: 10.1016/j.foodchem.2015.09.084 handle: http://hdl.handle.net/10449/26003 |
| Internal authors: | |
| Organization unit: | Department of Food Quality and Nutrition # CRI |
| Authors: | Arapitsas, P.; Della Corte, A.; Gika, H.; Narduzzi, L.; Mattivi, F.; Theodoridis, G. |
| Title: | Studying the effect of storage conditions on the metabolite content of red wine using HILIC LC–MS based metabolomics |
| Journal: | FOOD CHEMISTRY |
| Issue Date: | 2016 |
| Scientific Disciplinary Area: | Settore CHIM/10 - Chimica Degli Alimenti |
| Keywords ENG: | Wine metabolomics Holistic Wine conservation Method development Primary metabolities Metabolomics Normal phase LC QTOF MS HILIC Vitis vinifera |
| Language: | English |
| IF: | With Impact Factor ISI |
| Publication status: | Published |
| Nature of content: | Articolo in rivista/Article |
| Digital Object Identifier (DOI): | 10.1016/j.foodchem.2015.09.084 |
| URL: | http://authors.elsevier.com/a/1SArL16Ds1RUk0 |
| Appears in Collections: | 01 - Journal article |
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