The development of new resistant varieties to the oomycete Plasmopara viticola (Berk.& Curt) is a promising way to combat downy mildew (DM), one of the major diseases threatening the cultivated grapevine (Vitis vinifera L.). Taking advantage of a segregating population derived from “Merzling” (a mid-resistant hybrid) and “Teroldego” (a susceptible landrace), 136 F1 individuals were characterized by combining genetic, phenotypic, and gene expression data to elucidate the genetic basis of DM resistance and polyphenol biosynthesis upon P. viticola infection. An improved consensus linkage map was obtained by scoring 192 microsatellite markers. The progeny were screened for DM resistance and production of 42 polyphenols. QTL mapping showed that DM resistance is associated with the herein named Rpv3-3 specific haplotype and it identified 46 novel metabolic QTLs linked to 30 phenolics-related parameters. A list of the 95 most relevant candidate genes was generated by specifically exploring the stilbenoid-associated QTLs. Expression analysis of 11 genes in Rpv3-3+/− genotypes displaying disparity in DM resistance level and stilbenoid accumulation revealed significant new candidates for the genetic control of stilbenoid biosynthesis and oligomerization. These overall findings emphasized that DM resistance is likely mediated by the major Rpv3-3 haplotype and stilbenoid induction

Vezzulli, S.; Malacarne, G.; Masuero, D.; Vecchione, A.; Dolzani, C.; Goremykin, V.; Haile, Z.M.; Banchi, E.; Velasco, R.; Stefanini, M.; Vrhovsek, U.; Zulini, L.; Franceschi, P.; Moser, C. (2019). The Rpv3-3 haplotype and stilbenoid induction mediate downy mildew resistance in a grapevine interspecific population. FRONTIERS IN PLANT SCIENCE, 10: 234. doi: 10.3389/fpls.2019.00234 handle: http://hdl.handle.net/10449/51023

The Rpv3-3 haplotype and stilbenoid induction mediate downy mildew resistance in a grapevine interspecific population

Vezzulli, S.
Primo
;
Malacarne, G.
;
Masuero, D.;Vecchione, A.;Dolzani, Chiara;Goremykin, V.;Haile, Zeraye Mehari;Banchi, Elisa;Velasco, R.;Stefanini, M.;Vrhovsek, U.;Zulini, L.;Franceschi, P.;Moser, C.
Ultimo
2019-01-01

Abstract

The development of new resistant varieties to the oomycete Plasmopara viticola (Berk.& Curt) is a promising way to combat downy mildew (DM), one of the major diseases threatening the cultivated grapevine (Vitis vinifera L.). Taking advantage of a segregating population derived from “Merzling” (a mid-resistant hybrid) and “Teroldego” (a susceptible landrace), 136 F1 individuals were characterized by combining genetic, phenotypic, and gene expression data to elucidate the genetic basis of DM resistance and polyphenol biosynthesis upon P. viticola infection. An improved consensus linkage map was obtained by scoring 192 microsatellite markers. The progeny were screened for DM resistance and production of 42 polyphenols. QTL mapping showed that DM resistance is associated with the herein named Rpv3-3 specific haplotype and it identified 46 novel metabolic QTLs linked to 30 phenolics-related parameters. A list of the 95 most relevant candidate genes was generated by specifically exploring the stilbenoid-associated QTLs. Expression analysis of 11 genes in Rpv3-3+/− genotypes displaying disparity in DM resistance level and stilbenoid accumulation revealed significant new candidates for the genetic control of stilbenoid biosynthesis and oligomerization. These overall findings emphasized that DM resistance is likely mediated by the major Rpv3-3 haplotype and stilbenoid induction
Disease symptom phenotyping
“Merzling” Plasmopara viticola
Peroxidase
Polyphenols
QTL analysis
Stilbenes
Vitis spp.
Settore AGR/07 - GENETICA AGRARIA
2019
Vezzulli, S.; Malacarne, G.; Masuero, D.; Vecchione, A.; Dolzani, C.; Goremykin, V.; Haile, Z.M.; Banchi, E.; Velasco, R.; Stefanini, M.; Vrhovsek, U.; Zulini, L.; Franceschi, P.; Moser, C. (2019). The Rpv3-3 haplotype and stilbenoid induction mediate downy mildew resistance in a grapevine interspecific population. FRONTIERS IN PLANT SCIENCE, 10: 234. doi: 10.3389/fpls.2019.00234 handle: http://hdl.handle.net/10449/51023
File in questo prodotto:
File Dimensione Formato  
Vezzulli et al. _Front. Plant Sci. _March 2019.pdf

accesso aperto

Tipologia: Versione editoriale (Publisher’s layout)
Licenza: Creative commons
Dimensione 5.34 MB
Formato Adobe PDF
5.34 MB Adobe PDF Visualizza/Apri

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10449/51023
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 41
  • ???jsp.display-item.citation.isi??? 38
social impact