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ItalianoHypericum perforatum is known as an important medicinal plant, used for the treatment of several diseases, while its pharmacological properties are attributed to the presence of a wide range of secondary metabolites. Due to the great chemotypic variability of Hypericum species in the nature, and the demand for standardized herbal products, a detailed phytochemical investigation was carried out on different parts (herba, leaf, flowers) from wild collected and cultivated populations, using advanced chromatographic tools. Liquid Chromatographic analysis (LC-MS/MS MRM) revealed significant variability in the secondary metabolites content of the examined methanolic extracts. The most common derivatives belong to 9 groups i.e. benzoic acids, phenylpropanoids, coumarins, flavones, flavonols, flavan-3-ols, anthocyanins, phloroglucinols and naphtodianthrones. The main polyphenolic compounds were catechin, epicatechin, quercetin, quercetin 3-O-rhamnoside, quercetin 3-O-glucoside, neochlorogenic acid, proanthocyanidins (A and B series) and cyanidin-3-O-glucoside. In addition, the content of the characteristic compounds hypericin and hyperforin in herba crude extracts ranged between 0.5 and 1.7 mg/g and 0.6–3.3 mg/g respectively. The cytotoxic activity of the crude extracts was assessed at concentrations ranged between 0.01 and 100 μg/mL, on Caco-2 intestinal cancer cell cultures, and a cytotoxic behavior was shown only at the highest concentration of 100 μg/mL.
| Citation: | Sarrou, E.; Giassafaki, L.P.; Masuero, D.; Perenzoni, D.; Vizirianakis, I.S.; Irakli, M.; Chatzopoulou, P.; Martens, S. (2018). Metabolomics assisted fingerprint of Hypericum perforatum chemotypes and assessment of their cytotoxic activity. FOOD AND CHEMICAL TOXICOLOGY, 114: 325-333. doi: 10.1016/j.fct.2018.02.057 handle: http://hdl.handle.net/10449/46178 |
| Internal authors: | Martens, Stefan (Last) |
| Organization unit: | Department of Food Quality and Nutrition # CRI |
| Authors: | Sarrou, E.; Giassafaki, L.P.; Masuero, D.; Perenzoni, D.; Vizirianakis, I.S.; Irakli, M.; Chatzopoulou, P.; Martens, S. |
| Title: | Metabolomics assisted fingerprint of Hypericum perforatum chemotypes and assessment of their cytotoxic activity |
| Journal: | FOOD AND CHEMICAL TOXICOLOGY |
| Issue Date: | 2018 |
| Scientific Disciplinary Area: | Settore BIO/15 - Biologia Farmaceutica |
| Keywords ENG: | Hypericum perforatum Phenolics Anthocyanins Cytotoxicity Caco-2 cells |
| Language: | English |
| IF: | With Impact Factor ISI |
| Publication status: | Published |
| Nature of content: | Articolo in rivista/Article |
| Digital Object Identifier (DOI): | 10.1016/j.fct.2018.02.057 |
| Appears in Collections: | 01 - Journal article |
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