Drosophila suzukii Matsumura, an endemic pest in southeast Asia, has invaded Europe and the U.S.A. Unlike most of its closely related sibling species, the serrated ovipositor of D. suzukii permits ovipositing in undamaged fresh fruits. In the present study, volatiles are identified from host plants that are potentially involved in D. suzukii host recognition and oviposition behaviour. It is shown that mated females are attracted to volatiles emitted from intact fruits. The antennally-active suite of compounds released from the fresh fruits is identified by gas chromatography coupled with electroantennographic detection, as well as gas chromatography-mass spectrometry. In olfactometer bioassays, mated females are significantly attracted to an electroantennographically active volatile, isoamyl acetate, when tested at 10 μg of synthetic compound in a rubber septa, which has a release rate comparable to that of fresh fruits. In addition, a genomic survey shows that D. suzukii not only possesses the full repertoire of genes encoding odorant receptors activated by isoamyl acetate in D. melanogaster, but also that one of the genes, OR67a, is represented by five duplicated copies. These results indicate that D. suzukii uses olfactory cues to select oviposition sites. The identification of volatiles emitted by host fruits that attract D. suzukii may aid in the development of a selective and efficient synthetic lure for monitoring this pest. As a close relative of Drosophila melanogaster, D. suzukii provides a unique opportunity for understanding the physiological mechanisms involved in the shift of this species from use of rotten to ripe fruits for oviposition

Revadi, S.; Vitagliano, S.; Rossi Stacconi, M.V.; Ramasamy, S.; Mansourian, S.; Carlin, S.; Vrhovsek, U.; Becher, P.; Mazzoni, V.; Rota Stabelli, O.; Angeli, S.; Dekker, T.; Anfora, G. (2015). Olfactory responses of Drosophila suzukii females to host plant volatiles. PHYSIOLOGICAL ENTOMOLOGY, 40 (1): 54-64. doi: 10.1111/phen.12088 handle: http://hdl.handle.net/10449/24598

Olfactory responses of Drosophila suzukii females to host plant volatiles

Rossi Stacconi, Marco Valerio;Ramasamy, Sukanya;Carlin, Silvia;Vrhovsek, Urska;Mazzoni, Valerio;Rota Stabelli, Omar;Anfora, Gianfranco
2015-01-01

Abstract

Drosophila suzukii Matsumura, an endemic pest in southeast Asia, has invaded Europe and the U.S.A. Unlike most of its closely related sibling species, the serrated ovipositor of D. suzukii permits ovipositing in undamaged fresh fruits. In the present study, volatiles are identified from host plants that are potentially involved in D. suzukii host recognition and oviposition behaviour. It is shown that mated females are attracted to volatiles emitted from intact fruits. The antennally-active suite of compounds released from the fresh fruits is identified by gas chromatography coupled with electroantennographic detection, as well as gas chromatography-mass spectrometry. In olfactometer bioassays, mated females are significantly attracted to an electroantennographically active volatile, isoamyl acetate, when tested at 10 μg of synthetic compound in a rubber septa, which has a release rate comparable to that of fresh fruits. In addition, a genomic survey shows that D. suzukii not only possesses the full repertoire of genes encoding odorant receptors activated by isoamyl acetate in D. melanogaster, but also that one of the genes, OR67a, is represented by five duplicated copies. These results indicate that D. suzukii uses olfactory cues to select oviposition sites. The identification of volatiles emitted by host fruits that attract D. suzukii may aid in the development of a selective and efficient synthetic lure for monitoring this pest. As a close relative of Drosophila melanogaster, D. suzukii provides a unique opportunity for understanding the physiological mechanisms involved in the shift of this species from use of rotten to ripe fruits for oviposition
Drosophila suzukii
Kairomones
Drosophila suzukii
Cairomoni
Settore AGR/11 - ENTOMOLOGIA GENERALE E APPLICATA
2015
Revadi, S.; Vitagliano, S.; Rossi Stacconi, M.V.; Ramasamy, S.; Mansourian, S.; Carlin, S.; Vrhovsek, U.; Becher, P.; Mazzoni, V.; Rota Stabelli, O.; Angeli, S.; Dekker, T.; Anfora, G. (2015). Olfactory responses of Drosophila suzukii females to host plant volatiles. PHYSIOLOGICAL ENTOMOLOGY, 40 (1): 54-64. doi: 10.1111/phen.12088 handle: http://hdl.handle.net/10449/24598
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