Despite the increasing interest in using microbial-based technologies to support human space exploration, many unknowns remain not only on bioprocesses but also on microbial survivability and genetic stability under non-Earth conditions. Here the desert cyanobacterium Chroococcidiopsis sp. CCMEE 029 was investigated for robustness of the repair capability of DNA lesions accumulated under Mars-like conditions (UV radiation and atmosphere) simulated in low Earth orbit using the EXPOSE-R2 facility installed outside the International Space Station. Genomic alterations were determined in a space-derivate of Chroococcidiopsis sp. CCMEE 029 obtained upon reactivation on Earth of the space-exposed cells. Comparative analysis of whole-genome sequences showed no increased variant numbers in the space-derivate compared to triplicates of the reference strain maintained on the ground. This result advanced cyanobacteria-based technologies to support human space exploration

Napoli, A.; Micheletti, D.; Pindo, M.; Larger, S.; Cestaro, A.; de Vera, J.; Billi, D. (2022-05-19). Absence of increased genomic variants in the cyanobacterium Chroococcidiopsis exposed to Mars-like conditions outside the space station. SCIENTIFIC REPORTS, 12 (1): 8437. doi: 10.1038/s41598-022-12631-5 handle: https://hdl.handle.net/10449/78958

Absence of increased genomic variants in the cyanobacterium Chroococcidiopsis exposed to Mars-like conditions outside the space station

Micheletti, Diego;Pindo, Massimo;Larger, Simone;Cestaro, Alessandro;
2022-05-19

Abstract

Despite the increasing interest in using microbial-based technologies to support human space exploration, many unknowns remain not only on bioprocesses but also on microbial survivability and genetic stability under non-Earth conditions. Here the desert cyanobacterium Chroococcidiopsis sp. CCMEE 029 was investigated for robustness of the repair capability of DNA lesions accumulated under Mars-like conditions (UV radiation and atmosphere) simulated in low Earth orbit using the EXPOSE-R2 facility installed outside the International Space Station. Genomic alterations were determined in a space-derivate of Chroococcidiopsis sp. CCMEE 029 obtained upon reactivation on Earth of the space-exposed cells. Comparative analysis of whole-genome sequences showed no increased variant numbers in the space-derivate compared to triplicates of the reference strain maintained on the ground. This result advanced cyanobacteria-based technologies to support human space exploration
Settore BIO/19 - MICROBIOLOGIA GENERALE
19-mag-2022
Napoli, A.; Micheletti, D.; Pindo, M.; Larger, S.; Cestaro, A.; de Vera, J.; Billi, D. (2022-05-19). Absence of increased genomic variants in the cyanobacterium Chroococcidiopsis exposed to Mars-like conditions outside the space station. SCIENTIFIC REPORTS, 12 (1): 8437. doi: 10.1038/s41598-022-12631-5 handle: https://hdl.handle.net/10449/78958
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