Isoprene is the most abundant single biogenic volatile compound emitted by plants. Despite the relevance of this molecule to plant abiotic resistance and its impact on global atmospheric chemistry, little is known about the details of its mechanism of action. Here, we characterized through both physiological and molecular methods the mechanisms of action of isoprene using model transgenic arabidopsis lines overexpressing a monocot isoprene synthase gene. Our results demonstrated the eect that isoprene had on ABA signaling at dierent tissue-specific, spatial, and temporal scales. In particular, we found that isoprene enhanced stomatal sensitivity to ABA through upregulation of RD29B signaling gene. By contrast, isoprene decreased sensitivity to ABA in germinating seeds and roots, suggesting tissue-specific mechanisms of action. In leaves, isoprene caused the downregulation of COR15A and P5CS genes, suggesting that the enhanced tolerance to water-deprivation stress observed in isoprene-emitting plants may be mediated chiefly by an enhanced membrane integrity and tolerance to osmotic stress.

Xu, J.; Trainotti, L.; Li, M.; Varotto, C. (2020). Overexpression of isoprene synthase affects ABA- and drought-related gene expression and enhances tolerance to abiotic stress. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 21 (12): 4276. doi: 10.3390/ijms21124276 handle: http://hdl.handle.net/10449/64334

Overexpression of isoprene synthase affects ABA- and drought-related gene expression and enhances tolerance to abiotic stress

Xu, J.
Primo
;
Li, M.;Varotto, C.
Ultimo
2020-01-01

Abstract

Isoprene is the most abundant single biogenic volatile compound emitted by plants. Despite the relevance of this molecule to plant abiotic resistance and its impact on global atmospheric chemistry, little is known about the details of its mechanism of action. Here, we characterized through both physiological and molecular methods the mechanisms of action of isoprene using model transgenic arabidopsis lines overexpressing a monocot isoprene synthase gene. Our results demonstrated the eect that isoprene had on ABA signaling at dierent tissue-specific, spatial, and temporal scales. In particular, we found that isoprene enhanced stomatal sensitivity to ABA through upregulation of RD29B signaling gene. By contrast, isoprene decreased sensitivity to ABA in germinating seeds and roots, suggesting tissue-specific mechanisms of action. In leaves, isoprene caused the downregulation of COR15A and P5CS genes, suggesting that the enhanced tolerance to water-deprivation stress observed in isoprene-emitting plants may be mediated chiefly by an enhanced membrane integrity and tolerance to osmotic stress.
Isoprene
Abscisic acid (ABA)
Gene transcription
Water limitation stress
Isoprene synthase
Transgenic arabidopsis
Settore BIO/01 - BOTANICA GENERALE
2020
Xu, J.; Trainotti, L.; Li, M.; Varotto, C. (2020). Overexpression of isoprene synthase affects ABA- and drought-related gene expression and enhances tolerance to abiotic stress. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 21 (12): 4276. doi: 10.3390/ijms21124276 handle: http://hdl.handle.net/10449/64334
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10449/64334
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