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EnglishBiological interactions in the microbial communities of the rhizosphere continuously shape the gene expression patterns of each individual microorganism. A dual RNA-Seq approach was applied to obtain a comprehensive overview of the molecular mechanisms activated during the interaction between the biocontrol rhizobacterium Lysobacter capsici AZ78 and the soilborne phytopathogenic oomycete Phytophthora infestans. The RNA-Seq transcriptional profile of L. capsici AZ78 was characterized by up-regulation of genes concerned in the biogenesis of type 4 pilus and lytic enzymes, involved in host colonization and subsequent attack of the P. infestans cell wall, respectively. The activation of detoxification processes allowed L. capsici AZ78 to overcome the attempted defense processes of P. infestans. Moreover, the genes involved in antibiotic biosynthesis were up-regulated in L. capsici AZ78 and caused cell death in P. infestans, with the activation of putative apoptotic processes. The consequences of P. infestans cell death resulted in overall down-regulation of primary metabolic pathways, such as carbohydrate, nucleic acids and protein metabolisms. Overall, the mechanism of action of L. capsici AZ78 was related to parasitism and predatory activities that cause the death of P. infestans.
Tomada, S.; Sonego, P.; Moretto, M.; Engelen, K.; Pertot, I.; Perazzolli, M.; Puopolo, G. (2017). Dual RNA-Seq of Lysobacter capsici AZ78 – Phytophthora infestans interaction shows the implementation of attack strategies by the bacterium and unsuccessful oomycete defense responses. ENVIRONMENTAL MICROBIOLOGY, 19 (10): 4113-4125. doi: 10.1111/1462-2920.13861 handle: http://hdl.handle.net/10449/38285
| Citation: | Tomada, S.; Sonego, P.; Moretto, M.; Engelen, K.; Pertot, I.; Perazzolli, M.; Puopolo, G. (2017). Dual RNA-Seq of Lysobacter capsici AZ78 – Phytophthora infestans interaction shows the implementation of attack strategies by the bacterium and unsuccessful oomycete defense responses. ENVIRONMENTAL MICROBIOLOGY, 19 (10): 4113-4125. doi: 10.1111/1462-2920.13861 handle: http://hdl.handle.net/10449/38285 | |
| Internal authors: | TOMADA, SELENA (First) PERAZZOLLI, MICHELE (Second to last) PUOPOLO, GERARDO (Last) (Corresponding) | |
| Organization unit: | CRI [2011-01 - today]: DEPARTMENT OF SUSTAINABLE AGRO-ECOSYSTEMS AND BIORESOURCES CRI [2016-02 - today]: UNIT OF COMPUTATIONAL BIOLOGY | |
| Authors: | Tomada, S.; Sonego, P.; Moretto, M.; Engelen, K.; Pertot, I.; Perazzolli, M.; Puopolo, G. | |
| Title: | Dual RNA-Seq of Lysobacter capsici AZ78 – Phytophthora infestans interaction shows the implementation of attack strategies by the bacterium and unsuccessful oomycete defense responses | |
| Journal: | ENVIRONMENTAL MICROBIOLOGY | |
| Issue Date: | 2017 | |
| Date ahead of print: | 2017-08-14 | |
| Scientific Disciplinary Area: | Settore AGR/12 - PATOLOGIA VEGETALE | |
| Keywords ENG: | Lysobacter capsici Phytophthora infestans RNA-Seq Antibiotics Lytic enzymes Cell death | |
| Language: | English | |
| IF: | With Impact Factor ISI | |
| Publication status: | Published | |
| Nature of content: | Articolo in rivista/Article | |
| Digital Object Identifier (DOI): | http://dx.doi.org/10.1111/1462-2920.13861 | |
| EU grant: | Horizon2020 7° Programma quadro (FP7) | |
| EU grant agreement number: | 722642, 324416 | |
| Appears in Collections: | 01 - Journal article |
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