Metabolic surgery improves insulin resistance and type 2 diabetes possibly because of weight loss. We performed a novel sleeve gastrectomy in rats that resects ∼80% of the glandular portion, leaving the forestomach almost intact (glandular gastrectomy [GG]) and compared subsequent metabolic remodeling with a sham operation. GG did not affect body weight, at least after 10 weeks; improved hepatic and peripheral insulin sensitivity likely through increased Akt, glycogen synthase kinase 3, and AMPK phosphorylation; and reduced ectopic fat deposition and hepatic glycogen overaccumulation. Body adipose tissue was redistributed, with reduction of intraabdominal fat. We found a reduction of circulating ghrelin levels, increased GLP-1 plasma concentration, and remodeling of gut microbiome diversity characterized by a lower relative abundance of Ruminococcus and a higher relative abundance of Lactobacillus and Collinsella These data suggest that at least in rat, the glandular stomach plays a central role in the improvement of insulin resistance, even if obesity persists. GG provides a new model of the metabolically healthy obese phenotype.

Basso, N.; Soricelli, E.; Castagneto Gissey, L.; Casella, G.; Albanese, D.; Fava, F.; Donati, C.; Tuohy, K.M.; Angelini, G.; La Neve, F.; Severino, A.; Kamvissi Lorenz, V.; Birkenfeld, A.L.; Bornstein, S.; Manco, M.; Mingrone, G. (2016). Insulin resistance, microbiota, and fat distribution changes by a new model of vertical sleeve gastrectomy in obese rats. DIABETES, 65 (10): 2990-3001. doi: 10.2337/db16-0039 handle: http://hdl.handle.net/10449/37215

Insulin resistance, microbiota, and fat distribution changes by a new model of vertical sleeve gastrectomy in obese rats

Albanese, Davide;Fava, Francesca;Donati, Claudio;Tuohy, Kieran Michael;
2016-01-01

Abstract

Metabolic surgery improves insulin resistance and type 2 diabetes possibly because of weight loss. We performed a novel sleeve gastrectomy in rats that resects ∼80% of the glandular portion, leaving the forestomach almost intact (glandular gastrectomy [GG]) and compared subsequent metabolic remodeling with a sham operation. GG did not affect body weight, at least after 10 weeks; improved hepatic and peripheral insulin sensitivity likely through increased Akt, glycogen synthase kinase 3, and AMPK phosphorylation; and reduced ectopic fat deposition and hepatic glycogen overaccumulation. Body adipose tissue was redistributed, with reduction of intraabdominal fat. We found a reduction of circulating ghrelin levels, increased GLP-1 plasma concentration, and remodeling of gut microbiome diversity characterized by a lower relative abundance of Ruminococcus and a higher relative abundance of Lactobacillus and Collinsella These data suggest that at least in rat, the glandular stomach plays a central role in the improvement of insulin resistance, even if obesity persists. GG provides a new model of the metabolically healthy obese phenotype.
Settore MED/13 - ENDOCRINOLOGIA
2016
Basso, N.; Soricelli, E.; Castagneto Gissey, L.; Casella, G.; Albanese, D.; Fava, F.; Donati, C.; Tuohy, K.M.; Angelini, G.; La Neve, F.; Severino, A.; Kamvissi Lorenz, V.; Birkenfeld, A.L.; Bornstein, S.; Manco, M.; Mingrone, G. (2016). Insulin resistance, microbiota, and fat distribution changes by a new model of vertical sleeve gastrectomy in obese rats. DIABETES, 65 (10): 2990-3001. doi: 10.2337/db16-0039 handle: http://hdl.handle.net/10449/37215
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