The aim of the present study was to evaluate the antimicrobial activity of two synbiotic combinations, Lactobacillus fermentum with short-chain fructooligosaccharides (FOS-LF) and Bifidobacterium longum with isomaltooligosaccharides (IMO-BL), against enterohaemorrhagic Escherichia coli O157:H7 and enteropathogenic E. coli O86. Antimicrobial activity was determined (1) by co-culturing the synbiotics and pathogens in batch cultures, and (2) with the three-stage continuous culture system (gut model), inoculated with faecal slurry from an elderly donor. In the co-culture experiments, IMO-BL was significantly inhibitory to both E. coli strains, while FOS-LF was slightly inhibitory or not inhibitory. Factors other than acid production appeared to play a role in the inhibition. In the gut models, both synbiotics effectively inhibited E. coli O157 in the first vessel, but not in vessels 2 and 3. E. coli O86 was not significantly inhibited.
Likotrafiti, E.; Tuohy, K.M.; Gibson, G.R.; Rastall, R.A. (2016). Antimicrobial activity of selected synbiotics targeted for the elderly against pathogenic Escherichia coli strains. INTERNATIONAL JOURNAL OF FOOD SCIENCES AND NUTRITION, 67 (2): 83-91. doi: 10.3109/09637486.2015.1134444 handle: http://hdl.handle.net/10449/28337
Citation: | Likotrafiti, E.; Tuohy, K.M.; Gibson, G.R.; Rastall, R.A. (2016). Antimicrobial activity of selected synbiotics targeted for the elderly against pathogenic Escherichia coli strains. INTERNATIONAL JOURNAL OF FOOD SCIENCES AND NUTRITION, 67 (2): 83-91. doi: 10.3109/09637486.2015.1134444 handle: http://hdl.handle.net/10449/28337 |
Internal authors: | |
Organization unit: | Food Quality and Nutrition Department # CRI_2011-JAN2016 |
Authors: | Likotrafiti, E.; Tuohy, K.M.; Gibson, G.R.; Rastall, R.A. |
Title: | Antimicrobial activity of selected synbiotics targeted for the elderly against pathogenic Escherichia coli strains |
Journal: | INTERNATIONAL JOURNAL OF FOOD SCIENCES AND NUTRITION |
Issue Date: | 2016 |
Scientific Disciplinary Area: | Settore AGR/15 - Scienze E Tecnologie Alimentari |
Keywords ENG: | Bifidobacterium Lactobacillus Gut model |
Language: | English |
IF: | With Impact Factor ISI |
Publication status: | Published |
Nature of content: | Articolo in rivista/Article |
Digital Object Identifier (DOI): | http://dx.doi.org/10.3109/09637486.2015.1134444 |
Appears in Collections: | 01 - Journal article |
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