RATIONALE: The awareness of customers of the origin of foods has become an important issue. The growing demand for foods that are healthy, safe and of high quality has increased the need for traceability and clear labelling. Thus, this study investigates the capability of C and N stable isotope ratios to determine the geographical origin of several apple varieties grown in northern Italy. METHODS: Four apple varieties (Cripps Pink, Gala, Golden Delicious, Granny Smith) have been sampled in orchards located in the Districts of Bolzano, Ferrara, Verona and Udine (northern Italy). Carbon (δ13C) and nitrogen (δ15N) isotope values of the whole apple fruits and three sub-fractions (peel, pulp and seed) have been determined simultaneously by isotope ratio mass spectrometry. RESULTS: The δ13C and δ15N values of apples and apple sub-fractions, such as peel, seed and pulp, were significantly affected by the geographical origin and the fruit variety. The four varieties could be distinguished to a certain extent only within each district. A 99% correct identification of the samples according to their origin was, however, achieved by cross validation with the ’leave-one-out’ method. CONCLUSIONS: This study proves the potential of stable isotopes to discriminate the geographical origin of apples grown in orchards located only a few hundreds of kilometres apart. Stable isotopes were also able to discriminate different apple varieties, although only within small geographical areas.

Mimmo, T.; Camin, F.; Bontempo, L.; Capici, C.; Tagliavini, M.; Cesco, S.; Scampicchio, M. (2015). Traceability of different apple varieties by multivariate analysis of isotope ratio mass spectrometry data. RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 29 (21): 1984-1990. doi: 10.1002/rcm.7306 handle: http://hdl.handle.net/10449/26783

Traceability of different apple varieties by multivariate analysis of isotope ratio mass spectrometry data

Camin, Federica;Bontempo, Luana;
2015-01-01

Abstract

RATIONALE: The awareness of customers of the origin of foods has become an important issue. The growing demand for foods that are healthy, safe and of high quality has increased the need for traceability and clear labelling. Thus, this study investigates the capability of C and N stable isotope ratios to determine the geographical origin of several apple varieties grown in northern Italy. METHODS: Four apple varieties (Cripps Pink, Gala, Golden Delicious, Granny Smith) have been sampled in orchards located in the Districts of Bolzano, Ferrara, Verona and Udine (northern Italy). Carbon (δ13C) and nitrogen (δ15N) isotope values of the whole apple fruits and three sub-fractions (peel, pulp and seed) have been determined simultaneously by isotope ratio mass spectrometry. RESULTS: The δ13C and δ15N values of apples and apple sub-fractions, such as peel, seed and pulp, were significantly affected by the geographical origin and the fruit variety. The four varieties could be distinguished to a certain extent only within each district. A 99% correct identification of the samples according to their origin was, however, achieved by cross validation with the ’leave-one-out’ method. CONCLUSIONS: This study proves the potential of stable isotopes to discriminate the geographical origin of apples grown in orchards located only a few hundreds of kilometres apart. Stable isotopes were also able to discriminate different apple varieties, although only within small geographical areas.
Apples
Authentication
Geographical origin
Stable isotopes
IRMS
Classification
Settore CHIM/10 - CHIMICA DEGLI ALIMENTI
2015
Mimmo, T.; Camin, F.; Bontempo, L.; Capici, C.; Tagliavini, M.; Cesco, S.; Scampicchio, M. (2015). Traceability of different apple varieties by multivariate analysis of isotope ratio mass spectrometry data. RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 29 (21): 1984-1990. doi: 10.1002/rcm.7306 handle: http://hdl.handle.net/10449/26783
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