We have sequenced the genome of European pear, Pyrus communis cultivar ‘Bartlett’/‘William Bon Chrétien’ using second generation sequencing technology (Roche 454) A draft assembly was produced from single end reads, 2 kb, and 8 kb insert paired end reads using Newbler (version 2.7). The assembly contained 142,083 scaffolds greater than 499 bases (maximum scaffold length of 1.29Mb) covering a total of 577.3 Mb, representing 96.1% of the expected 600 Mb Pyrus genome. Preliminary analysis indicated that 549 SNP markers anchored 105 Mb (17.5%) of the assembled genome to a consensus genetic map of ‘Old Home’ x ‘Louise de Bonne Jersey’ and P019R054T042 x P037R048T081 segregating populations. Synteny with the apple genome primary assembly of ‘Golden Delicious’, and other rosaceous genomes is being used to extend putative anchoring. Gene prediction was performed and the use of the gene family of expansins will be reported as an example of assessment of the quality of the gene prediction. This ‘Bartlett’ genome sequence is a unique tool for identifying the genetic control of key horticultural traits and developing better pear cultivars, enabling wide application of marker‐assisted (MAS) and genomic selection (GS).

Gardiner, S.E.; Crowhurst, R.N.; Pindo, M.; Thrimawithana, A.; Storey, R.; Deng, C.; Dzierzon, H.; Fiers, M.; Lu, A.; Montanari, S.; Knaebel, M.; Saeed, M.; Kim, Y.K.; Ireland, I.; Allan, A.C.; Schaffer, R.; Cestaro, A.; Brewer, L.; Velasco, R.; Chagné, D. (2012). A draft genome sequence of European pear (Pyrus communis L. ‘Bartlett’). In: 6th Rosaceous Genomics Conference (RGC6), Mezzocorona (TN), 30th September-4th October 2012: 21. handle: http://hdl.handle.net/10449/21445

A draft genome sequence of European pear (Pyrus communis L. ‘Bartlett’)

Pindo, Massimo;Montanari, Sara;Cestaro, Alessandro;Velasco, Riccardo;
2012-01-01

Abstract

We have sequenced the genome of European pear, Pyrus communis cultivar ‘Bartlett’/‘William Bon Chrétien’ using second generation sequencing technology (Roche 454) A draft assembly was produced from single end reads, 2 kb, and 8 kb insert paired end reads using Newbler (version 2.7). The assembly contained 142,083 scaffolds greater than 499 bases (maximum scaffold length of 1.29Mb) covering a total of 577.3 Mb, representing 96.1% of the expected 600 Mb Pyrus genome. Preliminary analysis indicated that 549 SNP markers anchored 105 Mb (17.5%) of the assembled genome to a consensus genetic map of ‘Old Home’ x ‘Louise de Bonne Jersey’ and P019R054T042 x P037R048T081 segregating populations. Synteny with the apple genome primary assembly of ‘Golden Delicious’, and other rosaceous genomes is being used to extend putative anchoring. Gene prediction was performed and the use of the gene family of expansins will be reported as an example of assessment of the quality of the gene prediction. This ‘Bartlett’ genome sequence is a unique tool for identifying the genetic control of key horticultural traits and developing better pear cultivars, enabling wide application of marker‐assisted (MAS) and genomic selection (GS).
2012
Gardiner, S.E.; Crowhurst, R.N.; Pindo, M.; Thrimawithana, A.; Storey, R.; Deng, C.; Dzierzon, H.; Fiers, M.; Lu, A.; Montanari, S.; Knaebel, M.; Saeed, M.; Kim, Y.K.; Ireland, I.; Allan, A.C.; Schaffer, R.; Cestaro, A.; Brewer, L.; Velasco, R.; Chagné, D. (2012). A draft genome sequence of European pear (Pyrus communis L. ‘Bartlett’). In: 6th Rosaceous Genomics Conference (RGC6), Mezzocorona (TN), 30th September-4th October 2012: 21. handle: http://hdl.handle.net/10449/21445
File in questo prodotto:
File Dimensione Formato  
pag. 21.pdf

accesso aperto

Licenza: Tutti i diritti riservati (All rights reserved)
Dimensione 93.53 kB
Formato Adobe PDF
93.53 kB Adobe PDF Visualizza/Apri

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10449/21445
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus ND
  • ???jsp.display-item.citation.isi??? ND
social impact