In the present study the connection between the positional candidate gene VvDXS and muscat flavor was evaluated by investigating the expression profiles in the berries from a Muscat-type cultivar and a neutral cultivar and its nucleotide diversity of full ORF on grapevine accessions. The relationship between the transcription profile of VvDXS alleles and monoterpenoid content suggests that a particular trend in gene expression rather than the level of expression ratio affects monoterpenoid accumulation in ‘Moscato Bianco’. However a putative causal SNP responsible for a predicted non-neutral substitution was found to be significantly associated with muscat-flavored cultivars. Functional assays suggest that the putative causal SNP raises monoterpenoid accumulation by changing the 3D protein structure and by increasing VvDXS activity in Muscat enzyme form.
Battilana, J.; Emanuelli, F.; Costantini, L.; Gambino, G.; Gasperi, F.; Gribaudo, I.; This, P.; Boss, P.; Grando, M.S. (2011). Investigation of VvDXS function and its effects on muscat flavor levels. QUADERNI DI SCIENZE VITICOLE ED ENOLOGICHE..., 31 (2009/2010): 45-48. handle: http://hdl.handle.net/10449/19728
Investigation of VvDXS function and its effects on muscat flavor levels
Battilana, Juri;Emanuelli, Francesco;Costantini, Laura;Gasperi, Flavia;Grando, Maria Stella
2011-01-01
Abstract
In the present study the connection between the positional candidate gene VvDXS and muscat flavor was evaluated by investigating the expression profiles in the berries from a Muscat-type cultivar and a neutral cultivar and its nucleotide diversity of full ORF on grapevine accessions. The relationship between the transcription profile of VvDXS alleles and monoterpenoid content suggests that a particular trend in gene expression rather than the level of expression ratio affects monoterpenoid accumulation in ‘Moscato Bianco’. However a putative causal SNP responsible for a predicted non-neutral substitution was found to be significantly associated with muscat-flavored cultivars. Functional assays suggest that the putative causal SNP raises monoterpenoid accumulation by changing the 3D protein structure and by increasing VvDXS activity in Muscat enzyme form.File | Dimensione | Formato | |
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