Lemon [Citrus limon (L.) Burm. f.] is one of the most important citrus species, mainly prized for the organoleptic and nutraceutical properties of the juice and the peel (Muccilli et al., 2020). Since more than a century, mal secco, a tracheomycosis caused by the fungus Plenodomus tracheiphilus, represents a major threat for lemon cultivation in many Mediterranean countries, and mainly in Italy, causing the desiccation of twigs and branches leading, often, to the death of the plant (Catalano et al., 2021). To detect candidate genes linked to tolerance to mal secco, one inter- and one intra-specific segregating populations were obtained crossing two tolerant genotypes namely: ‘Interdonato’ lemon, and Citrus latipes, with the highly susceptible ‘Femminello Siracusano 2Kr’ lemon, generating a total of 240 seedlings. Two phenotyping methods were applied in parallel for two consecutive seasons: the artificial inoculation of the leaves and the in vivo assessment of symptoms through the cultivation in an area characterized by a high selective pressure of the pathogen. The genotyping was performed using a single-primer enrichment technology (SPET), leading to the definition of 49,478 robust SNPs; the known ancestors of lemon (citron, pummelo, mandarin and sour orange) were also genotyped to achieve a more complete picture of the allelic flow through generations. Marker-trait association analysis was carried out employing a pedigree-based approach (PBA) in which all accessions are analyzed simultaneously taking advantage of the availability of the pedigree records. Preliminary results highlighted the presence of a major QTLs in LG5. The understanding of the genetic regulation of the tolerance to mal secco and the availability of molecular markers linked to this trait will play a crucial role toward the obtainment of novel varieties coupling resistance to mal secco with optimal fruit quality and productivity
Di Guardo, M.; Catalano, C.; Troggio, M.; Catara, V.; Dimaria, G.; Sebastiano, S.; Martina, C.; Riccardo, R.; Arlotta, C.; Pietro Paolo, D.; Russo, G.; Di Silvestro, S.; Distefano, G.; Bianco, L.; Caruso, M.; La Malfa, S.; Gentile, A. (2024). Deciphering the genetic control of the tolerance to mal secco in lemon through a multi-parental QTL approach. ACTA HORTICULTURAE, 1412: 159-166. doi: 10.17660/actahortic.2024.1412.25 handle: https://hdl.handle.net/10449/88136
Deciphering the genetic control of the tolerance to mal secco in lemon through a multi-parental QTL approach
Troggio, M.;Bianco, L.;
2024-01-01
Abstract
Lemon [Citrus limon (L.) Burm. f.] is one of the most important citrus species, mainly prized for the organoleptic and nutraceutical properties of the juice and the peel (Muccilli et al., 2020). Since more than a century, mal secco, a tracheomycosis caused by the fungus Plenodomus tracheiphilus, represents a major threat for lemon cultivation in many Mediterranean countries, and mainly in Italy, causing the desiccation of twigs and branches leading, often, to the death of the plant (Catalano et al., 2021). To detect candidate genes linked to tolerance to mal secco, one inter- and one intra-specific segregating populations were obtained crossing two tolerant genotypes namely: ‘Interdonato’ lemon, and Citrus latipes, with the highly susceptible ‘Femminello Siracusano 2Kr’ lemon, generating a total of 240 seedlings. Two phenotyping methods were applied in parallel for two consecutive seasons: the artificial inoculation of the leaves and the in vivo assessment of symptoms through the cultivation in an area characterized by a high selective pressure of the pathogen. The genotyping was performed using a single-primer enrichment technology (SPET), leading to the definition of 49,478 robust SNPs; the known ancestors of lemon (citron, pummelo, mandarin and sour orange) were also genotyped to achieve a more complete picture of the allelic flow through generations. Marker-trait association analysis was carried out employing a pedigree-based approach (PBA) in which all accessions are analyzed simultaneously taking advantage of the availability of the pedigree records. Preliminary results highlighted the presence of a major QTLs in LG5. The understanding of the genetic regulation of the tolerance to mal secco and the availability of molecular markers linked to this trait will play a crucial role toward the obtainment of novel varieties coupling resistance to mal secco with optimal fruit quality and productivityFile | Dimensione | Formato | |
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