The search for new anti-infectives based on metal complexes is gaining momentum. Among the different options taken by researchers, the one involving the use of organometallic complexes is probably the most successful one with a compound, namely, ferroquine, already in clinical trials against malaria. In this study, we describe the preparation and in-depth characterization of 10 new (organometallic) derivatives of the approved antifungal drug fluconazole. Our rationale is that the sterol 14α-demethylase is an enzyme part of the ergosterol biosynthesis route in Trypanosoma and is similar to the one in pathogenic fungi. To demonstrate our postulate, docking experiments to assess the binding of our compounds with the enzyme were also performed. Our compounds were then tested on a range of fungal strains and parasitic organisms, including the protozoan parasite Trypanosoma cruzi (T. cruzi) responsible for Chagas disease, an endemic disease in Latin America that ranks among some of the most prevalent parasitic diseases worldwide. Of high interest, the two most potent compounds of the study on T. cruzi that contain a ferrocene or cobaltocenium were found to be harmless for an invertebrate animal model, namely, Caenorhabditis elegans (C. elegans), without affecting motility, viability, or development.

Lin, Y.; Scalese, G.; Bulman, C.A.; Vinck, R.; Blacque, O.; Paulino, M.; Ballesteros-Casallas, A.; Pérez Díaz, L.; Salinas, G.; Mitreva, M.; Weil, T.; Cariou, K.; Sakanari, J.A.; Gambino, D.; Gasser, G. (2024). Antifungal and antiparasitic activities of metallocene-containing fluconazole derivatives. ACS INFECTIOUS DISEASES, 10 (3): 938-950. doi: 10.1021/acsinfecdis.3c00577 handle: https://hdl.handle.net/10449/84175

Antifungal and antiparasitic activities of metallocene-containing fluconazole derivatives

Weil,T.
;
2024-01-01

Abstract

The search for new anti-infectives based on metal complexes is gaining momentum. Among the different options taken by researchers, the one involving the use of organometallic complexes is probably the most successful one with a compound, namely, ferroquine, already in clinical trials against malaria. In this study, we describe the preparation and in-depth characterization of 10 new (organometallic) derivatives of the approved antifungal drug fluconazole. Our rationale is that the sterol 14α-demethylase is an enzyme part of the ergosterol biosynthesis route in Trypanosoma and is similar to the one in pathogenic fungi. To demonstrate our postulate, docking experiments to assess the binding of our compounds with the enzyme were also performed. Our compounds were then tested on a range of fungal strains and parasitic organisms, including the protozoan parasite Trypanosoma cruzi (T. cruzi) responsible for Chagas disease, an endemic disease in Latin America that ranks among some of the most prevalent parasitic diseases worldwide. Of high interest, the two most potent compounds of the study on T. cruzi that contain a ferrocene or cobaltocenium were found to be harmless for an invertebrate animal model, namely, Caenorhabditis elegans (C. elegans), without affecting motility, viability, or development.
Antifungal agents
Bioorganometallic chemistry
Fluconazole
Metals in medicine
Chagas disease
Parasitic helminths
Neglected tropical diseases
Settore BIO/10 - BIOCHIMICA
2024
Lin, Y.; Scalese, G.; Bulman, C.A.; Vinck, R.; Blacque, O.; Paulino, M.; Ballesteros-Casallas, A.; Pérez Díaz, L.; Salinas, G.; Mitreva, M.; Weil, T.; Cariou, K.; Sakanari, J.A.; Gambino, D.; Gasser, G. (2024). Antifungal and antiparasitic activities of metallocene-containing fluconazole derivatives. ACS INFECTIOUS DISEASES, 10 (3): 938-950. doi: 10.1021/acsinfecdis.3c00577 handle: https://hdl.handle.net/10449/84175
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10449/84175
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