Fire blight, caused by Erwinia amylovora, is one of the most devastating apple diseases. The selection of cultivars of low susceptibility and the study of the genetic mechanisms of the disease play important roles in fire blight management. The susceptibility level to fire blight was evaluated in 102 accessions originating from Asturias, a cider-producing region located in the north of Spain with a wide apple germplasm. Evaluations took place under quarantine conditions using artificial inoculations of grafted plants. The results revealed wide variation in susceptibility responses and low-susceptible cultivars were identified. In addition, 91 cultivars were genotyped using the Affymetrix Axiom® Apple 480 K SNP array to conduct genome-wide association studies (GWAS). A statistically significant signal was detected on chromosome 10 using the multi-locus mixed model (MLMM). Two genes were identified as major putative candidate genes: a TIR-NBS-LRR class disease protein and a protein containing a development and cell death (DCD) domain. The outcomes of this study provide a promising source of information, particularly in the context of cider apples, and set a starting point for future genetic and breeding approaches

García-Fernández, B.; Dolcet-Sanjuan, R.; Micheletti, D.; Antón-Díaz, M.J.; Solsona, C.; Fernández, M.; Abad, X.; Dapena, E. (2023-12-04). Susceptibility evaluation to fire blight and genome-wide associations within a collection of Asturian apple accessions. PLANTS, 12 (23): 4068. doi: 10.3390/plants12234068 handle: https://hdl.handle.net/10449/83316

Susceptibility evaluation to fire blight and genome-wide associations within a collection of Asturian apple accessions

Micheletti, Diego;
2023-12-04

Abstract

Fire blight, caused by Erwinia amylovora, is one of the most devastating apple diseases. The selection of cultivars of low susceptibility and the study of the genetic mechanisms of the disease play important roles in fire blight management. The susceptibility level to fire blight was evaluated in 102 accessions originating from Asturias, a cider-producing region located in the north of Spain with a wide apple germplasm. Evaluations took place under quarantine conditions using artificial inoculations of grafted plants. The results revealed wide variation in susceptibility responses and low-susceptible cultivars were identified. In addition, 91 cultivars were genotyped using the Affymetrix Axiom® Apple 480 K SNP array to conduct genome-wide association studies (GWAS). A statistically significant signal was detected on chromosome 10 using the multi-locus mixed model (MLMM). Two genes were identified as major putative candidate genes: a TIR-NBS-LRR class disease protein and a protein containing a development and cell death (DCD) domain. The outcomes of this study provide a promising source of information, particularly in the context of cider apples, and set a starting point for future genetic and breeding approaches
Erwinia amylovora
Malus domestica
Apple bank germplasm
Apple breeding
Artificial inoculations
Cider apple
Genome-wide association studies
Local cultivars
Phenotypic evaluation
Putative candidate genes
Settore AGR/07 - GENETICA AGRARIA
4-dic-2023
García-Fernández, B.; Dolcet-Sanjuan, R.; Micheletti, D.; Antón-Díaz, M.J.; Solsona, C.; Fernández, M.; Abad, X.; Dapena, E. (2023-12-04). Susceptibility evaluation to fire blight and genome-wide associations within a collection of Asturian apple accessions. PLANTS, 12 (23): 4068. doi: 10.3390/plants12234068 handle: https://hdl.handle.net/10449/83316
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