Secretory immunoglobulin A (SIgA) interaction with commensal bacteria conditions microbiota composition and function. However, mechanisms regulating reciprocal control of microbiota and SIgA are not defined. Bacteria-derived adenosine triphosphate (ATP) limits T follicular helper (Tfh) cells in the Peyer's patches (PPs) via P2X7 receptor (P2X7R) and thereby SIgA generation. Here we show that hydrolysis of extracellular ATP (eATP) by apyrase results in amplification of the SIgA repertoire. The enhanced breadth of SIgA in mice colonized with apyrase-releasing Escherichia coli influences topographical distribution of bacteria and expression of genes involved in metabolic versus immune functions in the intestinal epithelium. SIgA-mediated conditioning of bacteria and enterocyte function is reflected by differences in nutrient absorption in mice colonized with apyrase-expressing bacteria. Apyrase-induced SIgA improves intestinal homeostasis and attenuates barrier impairment and susceptibility to infection by enteric pathogens in antibiotic-induced dysbiosis. Therefore, amplification of SIgA by apyrase can be leveraged to restore intestinal fitness in dysbiotic conditions

Perruzza, L.; Strati, F.; Raneri, M.; Li, H.; Gargari, G.; Rezzonico-Jost, T.; Palatella, M.; Kwee, I.; Morone, D.; Seehusen, F.; Sonego, P.; Donati, C.; Franceschi, P.; Macpherson, A.J.; Guglielmetti, S.; Greiff, V.; Grassi, F. (2022-07-19). Apyrase-mediated amplification of secretory IgA promotes intestinal homeostasis. CELL REPORTS, 40 (3): 111112. doi: 10.1016/j.celrep.2022.111112 handle: https://hdl.handle.net/10449/78964

Apyrase-mediated amplification of secretory IgA promotes intestinal homeostasis

Sonego, Paolo;Donati, Claudio;Franceschi, Pietro;
2022-07-19

Abstract

Secretory immunoglobulin A (SIgA) interaction with commensal bacteria conditions microbiota composition and function. However, mechanisms regulating reciprocal control of microbiota and SIgA are not defined. Bacteria-derived adenosine triphosphate (ATP) limits T follicular helper (Tfh) cells in the Peyer's patches (PPs) via P2X7 receptor (P2X7R) and thereby SIgA generation. Here we show that hydrolysis of extracellular ATP (eATP) by apyrase results in amplification of the SIgA repertoire. The enhanced breadth of SIgA in mice colonized with apyrase-releasing Escherichia coli influences topographical distribution of bacteria and expression of genes involved in metabolic versus immune functions in the intestinal epithelium. SIgA-mediated conditioning of bacteria and enterocyte function is reflected by differences in nutrient absorption in mice colonized with apyrase-expressing bacteria. Apyrase-induced SIgA improves intestinal homeostasis and attenuates barrier impairment and susceptibility to infection by enteric pathogens in antibiotic-induced dysbiosis. Therefore, amplification of SIgA by apyrase can be leveraged to restore intestinal fitness in dysbiotic conditions
CP: Immunology
T follicular helper cell
Dysbiosis
Extracellular ATP
Microbiota
Purinergic signaling
Secretory IgA
Settore MED/04 - PATOLOGIA GENERALE
19-lug-2022
Perruzza, L.; Strati, F.; Raneri, M.; Li, H.; Gargari, G.; Rezzonico-Jost, T.; Palatella, M.; Kwee, I.; Morone, D.; Seehusen, F.; Sonego, P.; Donati, C.; Franceschi, P.; Macpherson, A.J.; Guglielmetti, S.; Greiff, V.; Grassi, F. (2022-07-19). Apyrase-mediated amplification of secretory IgA promotes intestinal homeostasis. CELL REPORTS, 40 (3): 111112. doi: 10.1016/j.celrep.2022.111112 handle: https://hdl.handle.net/10449/78964
File in questo prodotto:
File Dimensione Formato  
2022 CR Franceschi.pdf

accesso aperto

Tipologia: Versione editoriale (Publisher’s layout)
Licenza: Creative commons
Dimensione 5.72 MB
Formato Adobe PDF
5.72 MB Adobe PDF Visualizza/Apri

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10449/78964
Citazioni
  • ???jsp.display-item.citation.pmc??? 2
  • Scopus 7
  • ???jsp.display-item.citation.isi??? 7
social impact