Arabidopsis is by far the plant model species most widely used for functional studies. The surface sterilization of Arabidopsis seeds is a fundamental step required towards this end. Thus, it is paramount to establish high-throughput Arabidopsis seed surface sterilization methods to handle tens to hundreds of samples (e.g., transgenic lines, ecotypes, or mutants) at once. A seed surface sterilization method based on the efficient elimination of liquid in tubes with a homemade suction device constructed from a common vacuum pump is presented in this study. By dramatically reducing labor-intensive hands-on time with this method handling several hundreds of samples in one day is possible with little effort. Series time-course analyses further indicated a highly flexible time range of surface sterilization by maintaining high germination rates. This method could be easily adapted for surface sterilization of other kinds of small seeds with simple customization of the suction device according to the seed size, and the speed desired to eliminate the liquid.

Li, M.; Yu, J.; Barbaro, E.; Varotto, C. (2021). High-throughput, robust and highly time-flexible method for surface sterilization of Arabidopsis seeds. JOURNAL OF VISUALIZED EXPERIMENTS, 176: e62893. doi: 10.3791/62893 handle: http://hdl.handle.net/10449/72218

High-throughput, robust and highly time-flexible method for surface sterilization of Arabidopsis seeds

Li, Mingai
Primo
;
Yu, Jiamei;Barbaro, Enrico;Varotto, Claudio
Ultimo
2021-01-01

Abstract

Arabidopsis is by far the plant model species most widely used for functional studies. The surface sterilization of Arabidopsis seeds is a fundamental step required towards this end. Thus, it is paramount to establish high-throughput Arabidopsis seed surface sterilization methods to handle tens to hundreds of samples (e.g., transgenic lines, ecotypes, or mutants) at once. A seed surface sterilization method based on the efficient elimination of liquid in tubes with a homemade suction device constructed from a common vacuum pump is presented in this study. By dramatically reducing labor-intensive hands-on time with this method handling several hundreds of samples in one day is possible with little effort. Series time-course analyses further indicated a highly flexible time range of surface sterilization by maintaining high germination rates. This method could be easily adapted for surface sterilization of other kinds of small seeds with simple customization of the suction device according to the seed size, and the speed desired to eliminate the liquid.
Settore BIO/01 - BOTANICA GENERALE
2021
Li, M.; Yu, J.; Barbaro, E.; Varotto, C. (2021). High-throughput, robust and highly time-flexible method for surface sterilization of Arabidopsis seeds. JOURNAL OF VISUALIZED EXPERIMENTS, 176: e62893. doi: 10.3791/62893 handle: http://hdl.handle.net/10449/72218
File in questo prodotto:
File Dimensione Formato  
Li_et_al_2021_jove.pdf

solo utenti autorizzati

Tipologia: Versione editoriale (Publisher’s layout)
Licenza: Tutti i diritti riservati (All rights reserved)
Dimensione 627.3 kB
Formato Adobe PDF
627.3 kB Adobe PDF   Visualizza/Apri   Richiedi una copia
Suppl_Figure_1.pdf

solo utenti autorizzati

Tipologia: Altro materiale allegato (Other attachments)
Licenza: Tutti i diritti riservati (All rights reserved)
Dimensione 3.87 MB
Formato Adobe PDF
3.87 MB Adobe PDF   Visualizza/Apri   Richiedi una copia

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10449/72218
Citazioni
  • ???jsp.display-item.citation.pmc??? 1
  • Scopus 1
  • ???jsp.display-item.citation.isi??? 1
social impact