Background Rain-induced fruit cracking is a major physiological problem in most sweet cherry cultivars. For an in vivo cracking assay, the ‘Christensen method’ (cracking evaluation following fruit immersion in water) is commonly used; however, this test does not adequately simulate environmental conditions. Herein, we have designed and evaluated a cracking protocol, named ‘Waterfall method’, in which fruits are continuously wetted under controlled conditions. Results The application of this method alone, or in combination with ‘Christensen method, was shown to be a reliable approach to characterize sweet cherry cracking behavior. Seventeen cherry cultivars were tested for their cracking behavior using both protocols, and primary as well as secondary metabolites identification was performed in skin tissue using a combined GC–MS and UPLC-MS/MS platform. Significant variations of some of the detected metabolites were discovered and important cracking index–metabolite correlations were identified. Conclusions We have established an alternative/complementary method of cherry cracking characterization alongside to Christiansen assay.

Michailidis, M.; Karagiannis, E.; Tanou, G.; Samiotaki, M.; Sarrou, E.; Karamanoli, K.; Lazaridou, A.; Martens, S.; Molassiotis, A. (2020). Sweet cherry fruit cracking: follow-up testing methods and cultivar-metabolic screening. PLANT METHODS, 16: 51. doi: 10.1186/s13007-020-00593-6 handle: http://hdl.handle.net/10449/60560

Sweet cherry fruit cracking: follow-up testing methods and cultivar-metabolic screening

Martens, S.;
2020-01-01

Abstract

Background Rain-induced fruit cracking is a major physiological problem in most sweet cherry cultivars. For an in vivo cracking assay, the ‘Christensen method’ (cracking evaluation following fruit immersion in water) is commonly used; however, this test does not adequately simulate environmental conditions. Herein, we have designed and evaluated a cracking protocol, named ‘Waterfall method’, in which fruits are continuously wetted under controlled conditions. Results The application of this method alone, or in combination with ‘Christensen method, was shown to be a reliable approach to characterize sweet cherry cracking behavior. Seventeen cherry cultivars were tested for their cracking behavior using both protocols, and primary as well as secondary metabolites identification was performed in skin tissue using a combined GC–MS and UPLC-MS/MS platform. Significant variations of some of the detected metabolites were discovered and important cracking index–metabolite correlations were identified. Conclusions We have established an alternative/complementary method of cherry cracking characterization alongside to Christiansen assay.
Cracking
Fruit
Primary metabolites
Secondary metabolites
Skin tissue
Sweet cherry cultivars
Settore BIO/04 - FISIOLOGIA VEGETALE
2020
Michailidis, M.; Karagiannis, E.; Tanou, G.; Samiotaki, M.; Sarrou, E.; Karamanoli, K.; Lazaridou, A.; Martens, S.; Molassiotis, A. (2020). Sweet cherry fruit cracking: follow-up testing methods and cultivar-metabolic screening. PLANT METHODS, 16: 51. doi: 10.1186/s13007-020-00593-6 handle: http://hdl.handle.net/10449/60560
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10449/60560
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