Sviluppo di metodiche NGS per la caratterizzazione di OGM non autorizzati: primi risultati

Current GMO detection systems have limited abilities to detect unauthorized genetically modified organisms (UGMs). One of the components of safety assessment is molecular characterization at DNA level, which helps to determine the copy number, integrity and stability of a transgene, characterize the integration site within the flanking regions. The emergence of next-genera on sequencing (NGS) technologies has provided highly sensitive and cost- and labor-effective alternative for molecular characterization compared to traditional analysis. The aim of the present study was to develop NGS techniques based on Illumina platform in order to identify UGMs and better characterize new GM events that can implement the information deriving from current laboratory analyses. The preliminary study of A5547-127 soy event showed that NGS was successful in characterizing the transgenic event and the surroundings of the insertion site.