Direct coupling of thin-layer chromatography (TLC) with matrix-assisted laser desorption ionization (MALDI) mass spectrometry allows fast and detailed characterization of a large variety of analytes. The use of this technique, however, presents great challenges in semiquantitative applications because of the complex phenomena occurring at the TLC surface. In our laboratory, we recently observed that the ion intensities of several alkali adduct ions were significantly different between the top and interior layer of the TLC plate. This indicates that the integrity of the TLC surface can have an important effect on the reproducibility of TLC- MALDI analyses. Graphical Abstract MALDI imaging reveals that surface integrity affects the detection of alkali adductions in TLC-MALDI
Dong, Y.; Ferrazza, R.; Anesi, A.; Guella, G.; Franceschi, P. (2017). TLC surface integrity affects the detection of alkali adduct ions in TLC-MALDI analysis. ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 409 (24): 5661-5666. doi: 10.1007/s00216-017-0501-9 handle: http://hdl.handle.net/10449/42883
TLC surface integrity affects the detection of alkali adduct ions in TLC-MALDI analysis
Dong, Y.Primo
;Anesi, A.;Franceschi, P.
Ultimo
2017-01-01
Abstract
Direct coupling of thin-layer chromatography (TLC) with matrix-assisted laser desorption ionization (MALDI) mass spectrometry allows fast and detailed characterization of a large variety of analytes. The use of this technique, however, presents great challenges in semiquantitative applications because of the complex phenomena occurring at the TLC surface. In our laboratory, we recently observed that the ion intensities of several alkali adduct ions were significantly different between the top and interior layer of the TLC plate. This indicates that the integrity of the TLC surface can have an important effect on the reproducibility of TLC- MALDI analyses. Graphical Abstract MALDI imaging reveals that surface integrity affects the detection of alkali adductions in TLC-MALDIFile | Dimensione | Formato | |
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