During the last decade, high-throughput genotyping has facilitated the dissection of complex traits in species with large/complex genomes and high level of genetic diversity. Array-based marker systems have been increasingly adopted for high-throughput genotyping, not only in model organisms, but also in many non-model plant species for which genomic resources are now available. A new high-density Affymetrix Axiom® SNP array has been built for the domesticated apple (Malus x domestica). It gathers more than 487K SNPs that are evenly distributed over the 17 chromosomes. The array has been built from the high-depth resequencing (~10-20x) of 63 different varieties covering most of the genetic diversity in cultivated apple. SNPs have been chosen by applying a focal points approach to enrich genic regions, but also to reach a uniform coverage of non-genic regions as to support SNP haplotype approaches. A total of 1324 apple accessions, including the 92 progenies of two mapping populations, have been genotyped with the Axiom®Apple480K to assess the effectiveness of the array. The majority of SNPs (359,994; 74%) fell in the most interesting class of Poly High Resolution polymorphisms. A novel filtering procedure was also devised to identify a subset of 275K robust markers that can be safely used for germplasm surveys in apple. A first application to genome wide association (GWA) study of two phenology traits (flowering time and maturity date) in six European germplasm collections is also presented. The Axiom®Apple480K has been publicly released and will likely be a reference tool for GWA studies in apple.
|Citation:||Bianco, L.; Muranty, H.; Cestaro, A.; Linsmith, G.; Denancé, C.; Théron, A.; Urrestarazu, J.; Poncet, C.; Micheletti, D.; Kersshbamer, E.; Di Pierro, E.A.; Larger, S.; Pindo, M.; van de Weg, E.; Davassi, A.; Laurens, F.; Tartarini, S.; Velasco, R.; Durel, C.E.; Troggio M. (2016). Development of the Axiom® Apple480K SNP genotyping array and its application for genome wide association study in apple. In: RGC8: 8th International Rosaceae Genomics Conference, Angers, France, 21-23 June 2016: 36 (S2P6). url: https://colloque.inra.fr/rgc8/Abstract-Book handle: http://hdl.handle.net/10449/34348|
|Organization unit:||Department of Genomics and Biology of Fruit Crops # CRI|
Unit of Computational Biology # CRI
|Authors:||Bianco, L.; Muranty, H.; Cestaro, A.; Linsmith, G.; Denancé, C.; Théron, A.; Urrestarazu, J.; Poncet, C.; Micheletti, D.; Kersshbamer, E.; Di Pierro, E.A.; Larger, S.; Pindo, M.; van de Weg, E.; Davassi, A.; Laurens, F.; Tartarini, S.; Velasco, R.; Durel, C.E.; Troggio M.|
|Title:||Development of the Axiom® Apple480K SNP genotyping array and its application for genome wide association study in apple|
|Scientific Disciplinary Area:||Settore AGR/07 - Genetica Agraria|
|Appears in Collections:||03 - Conference object|