The immune system is essential to maintain the mutualistic homeostatic interaction between the host and its micro- and mycobiota. Living as a commensal, Saccharomyces cerevisiae could potentially shape the immune response in a significant way. We observed that S. cerevisiae cells induce trained immunity in monocytes in a strain-dependent manner through enhanced TNFα and IL-6 production upon secondary stimulation with TLR ligands, as well as bacterial and fungal commensals. Differential chitin content accounts for the differences in training properties observed among strains, driving induction of trained immunity by increasing cytokine production and direct antimicrobial activity both in vitro and in vivo. These chitin-induced protective properties are intimately associated with its internalization, identifying a critical role of phagosome acidification to facilitate microbial digestion. This study reveals how commensal and passenger microorganisms could be important in promoting health and preventing mucosal diseases by modulating host defense towards pathogens and thus influencing the host microbiota-immune system interactions.
Rizzetto, L.; Ifrim, D.C.; Moretti, S.; Tocci, N.; Cheng, S.; Quintin, J.; Renga, G.; Oikonomou, V.; De Filippo, C.; Weil, T.F.A.L.; Blok, B.A.; Lenucci, M.S.; Santos, M.A.S.; Romani, L.; Netea, M.G.; Cavalieri, D. (2016). Fungal chitin induces trained immunity in human monocytes during cross-talk of the host with Saccharomyces cerevisiae. JOURNAL OF BIOLOGICAL CHEMISTRY, 291 (15): 7961-7972. doi: 10.1074/jbc.M115.699645 handle: http://hdl.handle.net/10449/32777
Fungal chitin induces trained immunity in human monocytes during cross-talk of the host with Saccharomyces cerevisiae
Rizzetto, Lisa;Tocci, Noemi;De Filippo, Carlotta;Weil, Tobias Franz Anton Ludwig;Cavalieri, Duccio
2016-01-01
Abstract
The immune system is essential to maintain the mutualistic homeostatic interaction between the host and its micro- and mycobiota. Living as a commensal, Saccharomyces cerevisiae could potentially shape the immune response in a significant way. We observed that S. cerevisiae cells induce trained immunity in monocytes in a strain-dependent manner through enhanced TNFα and IL-6 production upon secondary stimulation with TLR ligands, as well as bacterial and fungal commensals. Differential chitin content accounts for the differences in training properties observed among strains, driving induction of trained immunity by increasing cytokine production and direct antimicrobial activity both in vitro and in vivo. These chitin-induced protective properties are intimately associated with its internalization, identifying a critical role of phagosome acidification to facilitate microbial digestion. This study reveals how commensal and passenger microorganisms could be important in promoting health and preventing mucosal diseases by modulating host defense towards pathogens and thus influencing the host microbiota-immune system interactions.File | Dimensione | Formato | |
---|---|---|---|
J. Biol. Chem.-2016-Rizzetto-jbc.M115.699645.pdf
accesso aperto
Descrizione: Main article
Licenza:
Tutti i diritti riservati (All rights reserved)
Dimensione
1.09 MB
Formato
Adobe PDF
|
1.09 MB | Adobe PDF | Visualizza/Apri |
I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.