A high-throughput genotyping platform is needed to enable marker-assisted breeding in the allo-octoploid cultivated strawberry Fragaria × ananassa. Short-read sequences from one diploid and 19 octoploid accessions were aligned to the diploid Fragaria vesca 'Hawaii 4' reference genome to identify single nucleotide polymorphisms (SNPs) and indels for incorporation into a 90 K Affymetrix® Axiom® array. We report the development and preliminary evaluation of this array.
Bassil, N.V.; Davis, T.M.; Zhang, H.; Ficklin, S.; Mittmann, M.; Webster, T.; Mahoney, L.; Wood, D.; Alperin, E.S.; Rosyara, U.R.; Koehorst Vanc Putten, H.; Monfort, A.; Sargent, D.J.; Amaya, I.; Denoyes, B.; Bianco, L.; van Dijk, T.; Pirani, A.; Iezzoni, A.; Main, D.; Peace, C.; Yang, Y.; Whitaker, V.; Verma, S.; Bellon, L.; Brew, F.; Herrera, R.; van de Weg, E. (2015). Development and preliminary evaluation of a 90 K Axiom® SNP array for the allo-octoploid cultivated strawberry Fragaria × ananassa. BMC GENOMICS, 16 (155): 1-30. doi: 10.1186/s12864-015-1310-1 handle: http://hdl.handle.net/10449/32761
Development and preliminary evaluation of a 90 K Axiom® SNP array for the allo-octoploid cultivated strawberry Fragaria × ananassa
Sargent, Daniel James;Bianco, Luca;
2015-01-01
Abstract
A high-throughput genotyping platform is needed to enable marker-assisted breeding in the allo-octoploid cultivated strawberry Fragaria × ananassa. Short-read sequences from one diploid and 19 octoploid accessions were aligned to the diploid Fragaria vesca 'Hawaii 4' reference genome to identify single nucleotide polymorphisms (SNPs) and indels for incorporation into a 90 K Affymetrix® Axiom® array. We report the development and preliminary evaluation of this array.
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