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EnglishBackground: Fusarium oxysporum f.sp. radicis-lycopersici (FORL) is one of the most destructive necrotrophic pathogens affecting tomato crops, causing considerable field and greenhouse yield losses. Despite such major economic impact, little is known about the molecular mechanisms regulating Fusarium oxysporum f.sp. radicis-lycopersici resistance in tomato. Results: A transcriptomic experiment was carried out in order to investigate the main mechanisms of FORL response in resistant and susceptible isogenic tomato lines. Microarray analysis at 15 DPI (days post inoculum) revealed a distinct gene expression pattern between the two genotypes in the inoculated vs non-inoculated conditions. A model of plant response both for compatible and incompatible reactions was proposed. In particular, in the incompatible interaction an activation of defense genes related to secondary metabolite production and tryptophan metabolism was observed. Moreover, maintenance of the cell osmotic potential after the FORL challenging was mediated by a dehydrationinduced protein. As for the compatible interaction, activation of an oxidative burst mediated by peroxidases and a cytochrome monooxygenase induced cell degeneration and necrosis. Conclusions: Our work allowed comprehensive understanding of the molecular basis of the tomato-FORL interaction. The result obtained emphasizes a different transcriptional reaction between the resistant and the susceptible genotype to the FORL challenge. Our findings could lead to the improvement in disease control strategies.
Manzo, D.; Ferriello, F.; Puopolo, G.; Zoina, A.; D’Esposito, D.; Tardella, L.; Ferrarini, A.; Ercolano, M.R. (2016). Fusarium oxysporum f.sp. radicis-lycopersici induces distinct transcriptome reprogramming in resistant and susceptible isogenic tomato lines. BMC PLANT BIOLOGY, 16 (53): 1-14. doi: 10.1186/s12870-016-0740-5 handle: http://hdl.handle.net/10449/32725
| Citation: | Manzo, D.; Ferriello, F.; Puopolo, G.; Zoina, A.; D’Esposito, D.; Tardella, L.; Ferrarini, A.; Ercolano, M.R. (2016). Fusarium oxysporum f.sp. radicis-lycopersici induces distinct transcriptome reprogramming in resistant and susceptible isogenic tomato lines. BMC PLANT BIOLOGY, 16 (53): 1-14. doi: 10.1186/s12870-016-0740-5 handle: http://hdl.handle.net/10449/32725 |
| Internal authors: | |
| Organization unit: | Department of Sustainable Agro-ecosystems and Bioresources # CRI |
| Authors: | Manzo, D.; Ferriello, F.; Puopolo, G.; Zoina, A.; D’Esposito, D.; Tardella, L.; Ferrarini, A.; Ercolano, M.R. |
| Title: | Fusarium oxysporum f.sp. radicis-lycopersici induces distinct transcriptome reprogramming in resistant and susceptible isogenic tomato lines |
| Journal: | BMC PLANT BIOLOGY |
| Issue Date: | 2016 |
| Scientific Disciplinary Area: | Settore AGR/12 - Patologia Vegetale |
| Keywords ENG: | Solanum lycopersicum FORL resistance Necrotrophic pathogen Transcriptomic Callose deposition Dehydration-induced protein Oxidative burst Necrosis reaction |
| Language: | English |
| IF: | With Impact Factor ISI |
| Publication status: | Published |
| Nature of content: | Articolo in rivista/Article |
| Digital Object Identifier (DOI): | http://dx.doi.org/10.1186/s12870-016-0740-5 |
| Appears in Collections: | 01 - Journal article |
Files in This Item:
| File | Description | Type | License | |
|---|---|---|---|---|
| BMC Plant Biology 16_53.pdf | N/A |  | Open AccessView/Open |
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