RATIONALE: Desorption electrospray ionization (DESI) imaging is a fast analytical technique used to assess spatially resolved biological processes over unmodified sample surfaces. Although DESI profiling experiments have demonstrated that the properties of the sample surface significantly affect the outcomes of DESI analyses, the potential implications of these phenomena in imaging applications have not yet been explored extensively. METHODS: The distribution of endogenous and exogenous organic acids in pith and out pith region of grapevine stems was investigated by using DESI imaging, ion chromatography and direct infusion methods. Several common normalization strategies to account for the surface effect, including TIC normalization, addition of the internal standard in the spray solvent and deposition of the standard over the sample surface, were critically evaluated. RESULTS: DESI imaging results show that, in our case, the measured distributions of these small organic acids are not consistent with their 'true' localizations within the tissues. Furthermore, our results indicate that the common normalization strategies are not able to completely compensate for the observed surface effect. CONCLUSIONS: Variations in the tissue surface properties across the tissue sample can greatly affect the semi-quantitative detection of organic acids. Attention should be paid when interpreting DESI imaging results and an independent analytical validation step is important in untargeted DESI imaging investigations

Dong, Y.; Guella, G.; Franceschi, P. (2016). Impact of tissue surface properties on the desorption electrospray ionization imaging of organic acids in grapevine stem. RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 30 (6): 711-718. doi: 10.1002/rcm.7495 handle: http://hdl.handle.net/10449/28137

Impact of tissue surface properties on the desorption electrospray ionization imaging of organic acids in grapevine stem

Dong, Yonghui;Franceschi, Pietro
2016-01-01

Abstract

RATIONALE: Desorption electrospray ionization (DESI) imaging is a fast analytical technique used to assess spatially resolved biological processes over unmodified sample surfaces. Although DESI profiling experiments have demonstrated that the properties of the sample surface significantly affect the outcomes of DESI analyses, the potential implications of these phenomena in imaging applications have not yet been explored extensively. METHODS: The distribution of endogenous and exogenous organic acids in pith and out pith region of grapevine stems was investigated by using DESI imaging, ion chromatography and direct infusion methods. Several common normalization strategies to account for the surface effect, including TIC normalization, addition of the internal standard in the spray solvent and deposition of the standard over the sample surface, were critically evaluated. RESULTS: DESI imaging results show that, in our case, the measured distributions of these small organic acids are not consistent with their 'true' localizations within the tissues. Furthermore, our results indicate that the common normalization strategies are not able to completely compensate for the observed surface effect. CONCLUSIONS: Variations in the tissue surface properties across the tissue sample can greatly affect the semi-quantitative detection of organic acids. Attention should be paid when interpreting DESI imaging results and an independent analytical validation step is important in untargeted DESI imaging investigations
DESI
Grapevine
Mass spectrometry imaging
Settore CHIM/01 - CHIMICA ANALITICA
2016
Dong, Y.; Guella, G.; Franceschi, P. (2016). Impact of tissue surface properties on the desorption electrospray ionization imaging of organic acids in grapevine stem. RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 30 (6): 711-718. doi: 10.1002/rcm.7495 handle: http://hdl.handle.net/10449/28137
File in questo prodotto:
File Dimensione Formato  
2016 RCMS Franceschi.pdf

solo utenti autorizzati

Licenza: Tutti i diritti riservati (All rights reserved)
Dimensione 1.76 MB
Formato Adobe PDF
1.76 MB Adobe PDF   Visualizza/Apri   Richiedi una copia

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10449/28137
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 10
  • ???jsp.display-item.citation.isi??? 8
social impact