Lung specimens (n = 216) from six wildlife species were examined for occurrence of Pneumocystis species in pulmonary tissues. Among small mammals the shrew Sorex antinorii (80 %) were most frequently colonized. In contrast, foxes and badgers did not yield positive amplification. Host-specificity was noted, at least at the level of the host genus. Phylogenetic trees based on partial mtLSU and mtSSU showed high diversity of species corresponding to animal host diversity. Nuclear rDNA ITS data confirmed unambiguous separation of species. In conclusion, ITS is an excellent marker to distinguish species of the genus Pneumocystis
Danesi, P.; da Rold, G.; Rizzoli, A.; Hauffe, H.C.; Marangon, S.; Samerpitak, K.; Demanche, C.; Guillot, J.; Capelli, G.; de Hoog, S.G. (2016). Barcoding markers for Pneumocystis species in wildlife. FUNGAL BIOLOGY, 120 (2): 191-206. doi: 10.1016/j.funbio.2015.08.019 handle: http://hdl.handle.net/10449/27663
Barcoding markers for Pneumocystis species in wildlife
Rizzoli, Annapaola;Hauffe, Heidi Christine;
2016-01-01
Abstract
Lung specimens (n = 216) from six wildlife species were examined for occurrence of Pneumocystis species in pulmonary tissues. Among small mammals the shrew Sorex antinorii (80 %) were most frequently colonized. In contrast, foxes and badgers did not yield positive amplification. Host-specificity was noted, at least at the level of the host genus. Phylogenetic trees based on partial mtLSU and mtSSU showed high diversity of species corresponding to animal host diversity. Nuclear rDNA ITS data confirmed unambiguous separation of species. In conclusion, ITS is an excellent marker to distinguish species of the genus Pneumocystis
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