Direct delivery of purified Cas9 protein with guide RNA into plant cells, as opposed to plasmid-mediated delivery, displays high efficiency and reduced off-target effects. Following regeneration from edited cells, the ensuing plant is also likely to bypass genetically modified organism (GMO) legislation as the genome editing complex is degraded in the recipient cells.
Nagamangala Kanchiswamy, C.; Malnoy, M.A.; Velasco, R.; Kim, J.S.; Viola, R. (2015). Non-GMO genetically edited crop plant. TRENDS IN BIOTECHNOLOGY, 33 (9): 489-491. doi: 10.1016/j.tibtech.2015.04.002 handle: http://hdl.handle.net/10449/26530
Non-GMO genetically edited crop plant
Nagamangala Kanchiswamy, Chidananda;Malnoy, Mickael Arnaud;Velasco, Riccardo;Viola, Roberto
2015-01-01
Abstract
Direct delivery of purified Cas9 protein with guide RNA into plant cells, as opposed to plasmid-mediated delivery, displays high efficiency and reduced off-target effects. Following regeneration from edited cells, the ensuing plant is also likely to bypass genetically modified organism (GMO) legislation as the genome editing complex is degraded in the recipient cells.
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