Small RNA profiling and assessing its dependence on changing environmental factors have expanded our understanding of the transcriptional and post-transcriptional regulation of plant stress responses. Insufficient data have been documented earlier to depict the profiling of small RNA classes in temperature-associated stress which has a wide implication for climate change biology. In the present study, we report a comparative assessment of the genome-wide profiling of small RNAs in Arabidopsis thaliana using two conditional responses, induced by high- and low-temperature. Genome-wide profiling of small RNAs revealed an abundance of 21 nt small RNAs at low temperature, while high temperature showed an abundance of 21 nt and 24 nt small RNAs. The two temperature treatments altered the expression of a specific subset of mature miRNAs and displayed differential expression of a number of miRNA isoforms (isomiRs). Comparative analysis demonstrated that a large number of protein-coding genes can give rise to differentially expressed small RNAs following temperature shifts. Low temperature caused accumulation of small RNAs, corresponding to the sense strand of a number of cold-responsive genes. In contrast, high temperature stimulated the production of small RNAs of both polarities from genes encoding functionally diverse proteins

Baev, V.; Milev, I.; Naydenov, M.; Vachev, T.; Apostolova, E.; Mehterov, N.; Gozmanva, M.; Minkov, G.; Sablok, G.; Yahubyan, G. (2014). Insight into small RNA abundance and expression in high- and lowtemperaturestress response using deep sequencing in Arabidopsis. PLANT PHYSIOLOGY AND BIOCHEMISTRY, 84 (1): 105-114. doi: 10.1016/j.plaphy.2014.09.007 handle: http://hdl.handle.net/10449/25042

Insight into small RNA abundance and expression in high- and lowtemperature stress response using deep sequencing in Arabidopsis

Sablok, Gaurav;
2014-01-01

Abstract

Small RNA profiling and assessing its dependence on changing environmental factors have expanded our understanding of the transcriptional and post-transcriptional regulation of plant stress responses. Insufficient data have been documented earlier to depict the profiling of small RNA classes in temperature-associated stress which has a wide implication for climate change biology. In the present study, we report a comparative assessment of the genome-wide profiling of small RNAs in Arabidopsis thaliana using two conditional responses, induced by high- and low-temperature. Genome-wide profiling of small RNAs revealed an abundance of 21 nt small RNAs at low temperature, while high temperature showed an abundance of 21 nt and 24 nt small RNAs. The two temperature treatments altered the expression of a specific subset of mature miRNAs and displayed differential expression of a number of miRNA isoforms (isomiRs). Comparative analysis demonstrated that a large number of protein-coding genes can give rise to differentially expressed small RNAs following temperature shifts. Low temperature caused accumulation of small RNAs, corresponding to the sense strand of a number of cold-responsive genes. In contrast, high temperature stimulated the production of small RNAs of both polarities from genes encoding functionally diverse proteins
NGS
miRNAs
Small RNAs
Temperature stress
Arabidopsis
Settore BIO/11 - BIOLOGIA MOLECOLARE
2014
Baev, V.; Milev, I.; Naydenov, M.; Vachev, T.; Apostolova, E.; Mehterov, N.; Gozmanva, M.; Minkov, G.; Sablok, G.; Yahubyan, G. (2014). Insight into small RNA abundance and expression in high- and lowtemperaturestress response using deep sequencing in Arabidopsis. PLANT PHYSIOLOGY AND BIOCHEMISTRY, 84 (1): 105-114. doi: 10.1016/j.plaphy.2014.09.007 handle: http://hdl.handle.net/10449/25042
File in questo prodotto:
File Dimensione Formato  
1-s2.0-S0981942814002885-main.pdf

non disponibili

Licenza: Tutti i diritti riservati (All rights reserved)
Dimensione 1.58 MB
Formato Adobe PDF
1.58 MB Adobe PDF   Visualizza/Apri   Richiedi una copia

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10449/25042
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 33
  • ???jsp.display-item.citation.isi??? 30
social impact