A broad range fungal metagenomics study, using a Roche 454 pyrosequencing platform, was undertaken targeting internal transcribed spacer (ITS) fungal amplicons from six Norway spruce (Picea abies) forest soils in the northeastern Italian Alps. In our study, several stands having different bedrock composition (acid, basic, and intermediate) types, facing northern or southern slope exposure, and being in one of four stand age classes such as: gap, innovation, aggradation, and biostatic were investigated to examine the effect of these factors and their combinations on the associated fungal communities. The aim of the project was to assess the overall fungal diversity across a range of different conditions and to rank the shaping forces of different environmental factors over soil fungal community composition. In particular, the analysis determined the extent of conserved taxa and their site specific cities across a given geographic range and through a gradient of environmental conditions. More than 330 fungal taxa were identif ed. Results indicated patterns highly guided by pH as shown by a highly diverse community of acid/north spruce forest in which evenness was higher and successional stages were less evident and did not lead to emerging dominances at mature stages as instead occurred in each of the south-facing forests

La Porta, N.; Sablok, G.; Pindo, M.; Squartini, A. (2014). 454 Pyrosequencing analyses of mountain forest soils reveal a high fungal diversity and a rapid response to successional stages. In: Parrotta, J.E.; Moser, C.F.; Scherzer, A.J.; Koerth, N.E.; Lederle, D.R. (eds.) Sustaining forests, sustaining people: the role of research: XXIV IUFRO World Congress, 5-11 October 2014, Salt Lake City, USA: Commonwealth forestry association: 166. url: http://www.iufro.org/events/congresses/2014/ handle: http://hdl.handle.net/10449/24992

454 Pyrosequencing analyses of mountain forest soils reveal a high fungal diversity and a rapid response to successional stages

La Porta, Nicola;Sablok, Gaurav;Pindo, Massimo;
2014-01-01

Abstract

A broad range fungal metagenomics study, using a Roche 454 pyrosequencing platform, was undertaken targeting internal transcribed spacer (ITS) fungal amplicons from six Norway spruce (Picea abies) forest soils in the northeastern Italian Alps. In our study, several stands having different bedrock composition (acid, basic, and intermediate) types, facing northern or southern slope exposure, and being in one of four stand age classes such as: gap, innovation, aggradation, and biostatic were investigated to examine the effect of these factors and their combinations on the associated fungal communities. The aim of the project was to assess the overall fungal diversity across a range of different conditions and to rank the shaping forces of different environmental factors over soil fungal community composition. In particular, the analysis determined the extent of conserved taxa and their site specific cities across a given geographic range and through a gradient of environmental conditions. More than 330 fungal taxa were identif ed. Results indicated patterns highly guided by pH as shown by a highly diverse community of acid/north spruce forest in which evenness was higher and successional stages were less evident and did not lead to emerging dominances at mature stages as instead occurred in each of the south-facing forests
La Porta, N.; Sablok, G.; Pindo, M.; Squartini, A. (2014). 454 Pyrosequencing analyses of mountain forest soils reveal a high fungal diversity and a rapid response to successional stages. In: Parrotta, J.E.; Moser, C.F.; Scherzer, A.J.; Koerth, N.E.; Lederle, D.R. (eds.) Sustaining forests, sustaining people: the role of research: XXIV IUFRO World Congress, 5-11 October 2014, Salt Lake City, USA: Commonwealth forestry association: 166. url: http://www.iufro.org/events/congresses/2014/ handle: http://hdl.handle.net/10449/24992
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