Damage to various forest tree species caused by Diplodia sapinea has been reported in Italy since the early 20th century. However, until recently, systematic characterization of this microorganism, based on DNA sequence data, has been limited with only a general description of the fungus in the majority of the reports. To address this and provide a more accurate identification of Diplodia species associated on pine in Italy, we examined a total of 88 Botryosphaeriaceae isolates obtained from symptomatic and asymptomatic material from different hosts located in different geographical regions of Italy. Using molecular analysis, we were able to identify 67 isolates of D. sapinea and eight isolates of D. seriata. Diplodia sapinea was dominant on P. nigra shoots and was also detected on P. halepensis, P. pinea, P. pinaster, P. radiata and P. sylvestris, while D. seriata and other Botryosphaeriaceae appeared to be only occasionally present on shoots. The remaining 13 isolates represented nine different Botryosphaeriaceae species, occurring at low levels. Analysis of molecular variance (AMOVA) based on DAMD-PCR profiles detected low significant differences among D. sapinea isolates from the different sampling areas throughout Italy

Luchi, N.; Longa, C.M.O.; Danti, R.; Capretti, P.; Maresi, G. (2014). Diplodia sapinea: the main fungal species involved in the colonization of pine shootsin Italy. FOREST PATHOLOGY, 44 (5): 372-381. doi: 10.1111/efp.12109 handle: http://hdl.handle.net/10449/24250

Diplodia sapinea: the main fungal species involved in the colonization of pine shoots in Italy

Longa, Claudia Maria Oliveira;Maresi, Giorgio
2014-01-01

Abstract

Damage to various forest tree species caused by Diplodia sapinea has been reported in Italy since the early 20th century. However, until recently, systematic characterization of this microorganism, based on DNA sequence data, has been limited with only a general description of the fungus in the majority of the reports. To address this and provide a more accurate identification of Diplodia species associated on pine in Italy, we examined a total of 88 Botryosphaeriaceae isolates obtained from symptomatic and asymptomatic material from different hosts located in different geographical regions of Italy. Using molecular analysis, we were able to identify 67 isolates of D. sapinea and eight isolates of D. seriata. Diplodia sapinea was dominant on P. nigra shoots and was also detected on P. halepensis, P. pinea, P. pinaster, P. radiata and P. sylvestris, while D. seriata and other Botryosphaeriaceae appeared to be only occasionally present on shoots. The remaining 13 isolates represented nine different Botryosphaeriaceae species, occurring at low levels. Analysis of molecular variance (AMOVA) based on DAMD-PCR profiles detected low significant differences among D. sapinea isolates from the different sampling areas throughout Italy
Diplodia sapinea
Shoot blight
Settore AGR/12 - PATOLOGIA VEGETALE
2014
Luchi, N.; Longa, C.M.O.; Danti, R.; Capretti, P.; Maresi, G. (2014). Diplodia sapinea: the main fungal species involved in the colonization of pine shootsin Italy. FOREST PATHOLOGY, 44 (5): 372-381. doi: 10.1111/efp.12109 handle: http://hdl.handle.net/10449/24250
File in questo prodotto:
File Dimensione Formato  
Dsapinea&pine.pdf

solo utenti autorizzati

Licenza: Tutti i diritti riservati (All rights reserved)
Dimensione 1.02 MB
Formato Adobe PDF
1.02 MB Adobe PDF   Visualizza/Apri   Richiedi una copia

Questo articolo è pubblicato sotto una Licenza Licenza Creative Commons Creative Commons

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10449/24250
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 47
  • ???jsp.display-item.citation.isi??? 41
social impact