One of the main hurdles for the development of an effective and broadly protective vaccine against non-encapsulated isolates of Haemophilus influenzae (NTHi) lies in the genetic diversity of the species, which renders extremely difficult the identification of cross-protective candidate antigens. To assess whether a population structure of NTHi could be defined, we performed genome sequencing of a collection of diverse clinical isolates representative of both carriage and disease and of the diversity of the natural population. Analysis of the distribution of polymorphic sites in the core genome and of the composition of the accessory genome defined distinct evolutionary clades and supported a predominantly clonal evolution of NTHi, with the majority of genetic information transmitted vertically within lineages. A correlation between the population structure and the presence of selected surface-associated proteins and lipooligosaccharide structure, known to contribute to virulence, was found. This high-resolution, genome-based population structure of NTHi provides the foundation to obtain a better understanding of NTHi adaptation to the host, as well as its commensal and virulence behavior, that could facilitate intervention strategies against disease caused by this important human pathogen

De Chiara, M.; Hood, D.; Muzzi, A.; Pickard, D.J.; Perkins, T.; Pizza, M.; Dougan, G.; Rappuoli, R.; Moxon, E.R.; Soriani, M.; Donati, C. (2014). Genome sequencing of disease and carriage isolates of nontypeable Haemophilus influenzae identifies discrete population structure. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 111 (14): 5439-5444. doi: 10.1073/pnas.1403353111 handle: http://hdl.handle.net/10449/23994

Genome sequencing of disease and carriage isolates of nontypeable Haemophilus influenzae identifies discrete population structure

Donati, Claudio
2014-01-01

Abstract

One of the main hurdles for the development of an effective and broadly protective vaccine against non-encapsulated isolates of Haemophilus influenzae (NTHi) lies in the genetic diversity of the species, which renders extremely difficult the identification of cross-protective candidate antigens. To assess whether a population structure of NTHi could be defined, we performed genome sequencing of a collection of diverse clinical isolates representative of both carriage and disease and of the diversity of the natural population. Analysis of the distribution of polymorphic sites in the core genome and of the composition of the accessory genome defined distinct evolutionary clades and supported a predominantly clonal evolution of NTHi, with the majority of genetic information transmitted vertically within lineages. A correlation between the population structure and the presence of selected surface-associated proteins and lipooligosaccharide structure, known to contribute to virulence, was found. This high-resolution, genome-based population structure of NTHi provides the foundation to obtain a better understanding of NTHi adaptation to the host, as well as its commensal and virulence behavior, that could facilitate intervention strategies against disease caused by this important human pathogen
Genomics
Microbiology
Population genetics
NTHi
Genomica
Microbiologia
NTHi
Settore BIO/18 - GENETICA
2014
De Chiara, M.; Hood, D.; Muzzi, A.; Pickard, D.J.; Perkins, T.; Pizza, M.; Dougan, G.; Rappuoli, R.; Moxon, E.R.; Soriani, M.; Donati, C. (2014). Genome sequencing of disease and carriage isolates of nontypeable Haemophilus influenzae identifies discrete population structure. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 111 (14): 5439-5444. doi: 10.1073/pnas.1403353111 handle: http://hdl.handle.net/10449/23994
File in questo prodotto:
File Dimensione Formato  
DeChiara_PNAS_2014.pdf

accesso aperto

Descrizione: Main article
Licenza: Tutti i diritti riservati (All rights reserved)
Dimensione 2.75 MB
Formato Adobe PDF
2.75 MB Adobe PDF Visualizza/Apri

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10449/23994
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 87
  • ???jsp.display-item.citation.isi??? 83
social impact