Ljungan virus (LV, genus Parechovirus, family Picornaviridae) is considered currently to be a rodent-borne virus. Despite suggested human disease associations, its zoonotic potential remains unclear. To date, LV antibody prevalence in both humans and rodents has not been studied. In this study, two different LV immunofluorescence assays (LV IFAs) were developed with LV genotypes 1 (LV strain 87-012G) and 2 (LV strain 145SLG), and crossneutralization and -reaction studies were carried out with LV strain 145SLG. Finally, a panel of 37 Finnish sera was screened for anti-LV antibodies using two different LV IFAs (LV 145SLG and LV 87-012G) and a neutralization (NT) assay (LV 145SLG), and 50 samples from Myodes glareolus by LV IFA (LV 145SLG). The LV seroprevalence study showed 38% and 18% positivity in humans and M. glareolus, respectively. LV IFAs and NT assays were compared, and the results were in good agreement. The data are the first evidence of humans and rodents coming into contact with LV in Finland. Additional studies are required in order to acquire a better understanding of the prevalence, epidemiological patterns and possible disease association of LV infections.

Jääskeläinen, A.J.; Kolehmainen, P.; Voutilainen, L.; Hauffe, H.C.; Kallio Kokko, H.; Lappalainen, M.; Tolf, C.; Lindberg, A.M.; Henttonen, H.; Vaheri, A.; Tauriainen, S.; Vapalati, O. (2013). Evidence of Ljungan Virus specific antibodies in humans and rodents, Finland. JOURNAL OF MEDICAL VIROLOGY, 85 (11): 2001-2008. doi: 10.1002/jmv.23681 handle: http://hdl.handle.net/10449/22350

Evidence of Ljungan Virus specific antibodies in humans and rodents, Finland

Hauffe, Heidi Christine;
2013-01-01

Abstract

Ljungan virus (LV, genus Parechovirus, family Picornaviridae) is considered currently to be a rodent-borne virus. Despite suggested human disease associations, its zoonotic potential remains unclear. To date, LV antibody prevalence in both humans and rodents has not been studied. In this study, two different LV immunofluorescence assays (LV IFAs) were developed with LV genotypes 1 (LV strain 87-012G) and 2 (LV strain 145SLG), and crossneutralization and -reaction studies were carried out with LV strain 145SLG. Finally, a panel of 37 Finnish sera was screened for anti-LV antibodies using two different LV IFAs (LV 145SLG and LV 87-012G) and a neutralization (NT) assay (LV 145SLG), and 50 samples from Myodes glareolus by LV IFA (LV 145SLG). The LV seroprevalence study showed 38% and 18% positivity in humans and M. glareolus, respectively. LV IFAs and NT assays were compared, and the results were in good agreement. The data are the first evidence of humans and rodents coming into contact with LV in Finland. Additional studies are required in order to acquire a better understanding of the prevalence, epidemiological patterns and possible disease association of LV infections.
Ljungan virus
Neutralization
Seroprevalence
Settore BIO/13 - BIOLOGIA APPLICATA
2013
Jääskeläinen, A.J.; Kolehmainen, P.; Voutilainen, L.; Hauffe, H.C.; Kallio Kokko, H.; Lappalainen, M.; Tolf, C.; Lindberg, A.M.; Henttonen, H.; Vaheri, A.; Tauriainen, S.; Vapalati, O. (2013). Evidence of Ljungan Virus specific antibodies in humans and rodents, Finland. JOURNAL OF MEDICAL VIROLOGY, 85 (11): 2001-2008. doi: 10.1002/jmv.23681 handle: http://hdl.handle.net/10449/22350
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10449/22350
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