Escherichia coli K12 is a commensal bacteria and one of the best-studied model organisms. Salmonella enterica serovar Typhimurium, on the other hand, is a facultative intracellular pathogen. These two prokaryotic species can be considered related phylogenetically, and they share a large amount of their genetic material, which is commonly termed the "core genome." Despite their shared core genome, both species display very different lifestyles, and it is unclear to what extent the core genome, apart from the species-specific genes, plays a role in this lifestyle divergence. In this study, we focus on the differences in expression domains for the orthologous genes in E. coli and S. Typhimurium. The iterative comparison of coexpression methodology was used on large expression compendia of both species to uncover the conservation and divergence of gene expression. We found that gene expression conservation occurs mostly independently from amino acid similarity. According to our estimates, at least more than one quarter of the orthologous genes has a different expression domain in E. coli than in S. Typhimurium. Genes involved with key cellular processes are most likely to have conserved their expression domains, whereas genes showing diverged expression are associated with metabolic processes that, although present in both species, are regulated differently. The expression domains of the shared "core" genome of E. coli and S. Typhimurium, consisting of highly conserved orthologs, have been tuned to help accommodate the differences in lifestyle and the pathogenic potential of Salmonella

Meysman, P.; Sanchez Rodriguez, A.; Fu, Q.; Marchal, K.; Engelen, K.A. (2013). Expression divergence between Escherichia coli and Salmonella enterica serovar Typhimurium reflects their lifestyles. MOLECULAR BIOLOGY AND EVOLUTION, 30 (6): 1302-1314. doi: 10.1093/molbev/mst029 handle: http://hdl.handle.net/10449/22221

Expression divergence between Escherichia coli and Salmonella enterica serovar Typhimurium reflects their lifestyles

Engelen, Kristof Arthur
2013-01-01

Abstract

Escherichia coli K12 is a commensal bacteria and one of the best-studied model organisms. Salmonella enterica serovar Typhimurium, on the other hand, is a facultative intracellular pathogen. These two prokaryotic species can be considered related phylogenetically, and they share a large amount of their genetic material, which is commonly termed the "core genome." Despite their shared core genome, both species display very different lifestyles, and it is unclear to what extent the core genome, apart from the species-specific genes, plays a role in this lifestyle divergence. In this study, we focus on the differences in expression domains for the orthologous genes in E. coli and S. Typhimurium. The iterative comparison of coexpression methodology was used on large expression compendia of both species to uncover the conservation and divergence of gene expression. We found that gene expression conservation occurs mostly independently from amino acid similarity. According to our estimates, at least more than one quarter of the orthologous genes has a different expression domain in E. coli than in S. Typhimurium. Genes involved with key cellular processes are most likely to have conserved their expression domains, whereas genes showing diverged expression are associated with metabolic processes that, although present in both species, are regulated differently. The expression domains of the shared "core" genome of E. coli and S. Typhimurium, consisting of highly conserved orthologs, have been tuned to help accommodate the differences in lifestyle and the pathogenic potential of Salmonella
Gene expression
Expression divergence
Expression conservation
Salmonella
Pathogenesis
Escherichia coli
Settore BIO/18 - GENETICA
Meysman, P.; Sanchez Rodriguez, A.; Fu, Q.; Marchal, K.; Engelen, K.A. (2013). Expression divergence between Escherichia coli and Salmonella enterica serovar Typhimurium reflects their lifestyles. MOLECULAR BIOLOGY AND EVOLUTION, 30 (6): 1302-1314. doi: 10.1093/molbev/mst029 handle: http://hdl.handle.net/10449/22221
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