In plants, transgenes are generally more sensitive against RNA silencing than endogenes are. In this study, we generated a transgene that structurally mimicks an endogene. It is composed of endogenous promoter, 5'-UTR, introns, 3'-UTR and terminator elements. Our data revealed that, in contrast to a conventional transgene, an endogene-resembling transgene was more stably expressed and poorly processed into small RNAs. In addition, although both constructs triggered methylation of homologous DNA sequences at similar levels, the endogene-resembling transgene exhibited significantly delayed onset of local and systemic silencing

Dadami, E.; Moser, M.; Zwiebel, M.; Krczal, G.; Wassenegger, M.; Dalakouras, A. (2013). An endogene-resembling transgene delays the onset of silencing and limits siRNA accumulation. FEBS LETTERS, 587 (6): 706-710. doi: 10.1016/j.febslet.2013.01.045 handle: http://hdl.handle.net/10449/21891

An endogene-resembling transgene delays the onset of silencing and limits siRNA accumulation

Moser, Mirko;
2013-01-01

Abstract

In plants, transgenes are generally more sensitive against RNA silencing than endogenes are. In this study, we generated a transgene that structurally mimicks an endogene. It is composed of endogenous promoter, 5'-UTR, introns, 3'-UTR and terminator elements. Our data revealed that, in contrast to a conventional transgene, an endogene-resembling transgene was more stably expressed and poorly processed into small RNAs. In addition, although both constructs triggered methylation of homologous DNA sequences at similar levels, the endogene-resembling transgene exhibited significantly delayed onset of local and systemic silencing
RNA silencing
Small RNA
GFP
DNA methylation
Nicotiana benthamiana
Settore AGR/07 - GENETICA AGRARIA
2013
Dadami, E.; Moser, M.; Zwiebel, M.; Krczal, G.; Wassenegger, M.; Dalakouras, A. (2013). An endogene-resembling transgene delays the onset of silencing and limits siRNA accumulation. FEBS LETTERS, 587 (6): 706-710. doi: 10.1016/j.febslet.2013.01.045 handle: http://hdl.handle.net/10449/21891
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10449/21891
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