Variation in protein sequence and gene expression each contribute to phenotypic diversity, and may be subject to similar selective pressures. Eusocial insects are particularly useful for investigating the evolutionary link between protein sequence and condition-dependent patterns of gene expression because gene expression plays a central role in determining differences between eusocial insect sexes and castes. We investigated the relationship between protein coding sequence evolution and gene expression patterns in the fire ants Solenopsis invicta, S. richteri, and their hybrids to gain greater insight into how selection jointly operates on gene expression and coding sequence. We found that genes with high expression variability within castes and sexes were frequently differentially expressed between castes and sexes, as well as between species and hybrids. These results indicate that genes showing high variation in expression in one context also tend to show high variation in expression in other contexts. Our analyses further revealed that variation in both intra- and interspecific gene expression was positively associated with rate of protein sequence evolution in Solenopsis. This suggests that selective constraints on a gene operate both at the level of protein sequence and at the level of gene expression regulation. Overall, our study provides one of the strongest demonstrations that selective constraints mediate both protein sequence evolution and gene expression variability across different biological contexts and timescales.

Hunt, B.G.; Ometto, L.; Keller, L.; Goodisman, M.A.D. (2013). Evolution at two levels in fire ants: the relationship between patterns of gene expression and protein sequence evolution. MOLECULAR BIOLOGY AND EVOLUTION, 30 (2): 263-271. doi: 10.1093/molbev/mss234 handle: http://hdl.handle.net/10449/21745

Evolution at two levels in fire ants: the relationship between patterns of gene expression and protein sequence evolution

Ometto, Lino;
2013-01-01

Abstract

Variation in protein sequence and gene expression each contribute to phenotypic diversity, and may be subject to similar selective pressures. Eusocial insects are particularly useful for investigating the evolutionary link between protein sequence and condition-dependent patterns of gene expression because gene expression plays a central role in determining differences between eusocial insect sexes and castes. We investigated the relationship between protein coding sequence evolution and gene expression patterns in the fire ants Solenopsis invicta, S. richteri, and their hybrids to gain greater insight into how selection jointly operates on gene expression and coding sequence. We found that genes with high expression variability within castes and sexes were frequently differentially expressed between castes and sexes, as well as between species and hybrids. These results indicate that genes showing high variation in expression in one context also tend to show high variation in expression in other contexts. Our analyses further revealed that variation in both intra- and interspecific gene expression was positively associated with rate of protein sequence evolution in Solenopsis. This suggests that selective constraints on a gene operate both at the level of protein sequence and at the level of gene expression regulation. Overall, our study provides one of the strongest demonstrations that selective constraints mediate both protein sequence evolution and gene expression variability across different biological contexts and timescales.
Caste polyphenism
Hybrid incompatibility
Selective constraint
Sexual dimorphism
Social insect
Settore BIO/18 - GENETICA
Hunt, B.G.; Ometto, L.; Keller, L.; Goodisman, M.A.D. (2013). Evolution at two levels in fire ants: the relationship between patterns of gene expression and protein sequence evolution. MOLECULAR BIOLOGY AND EVOLUTION, 30 (2): 263-271. doi: 10.1093/molbev/mss234 handle: http://hdl.handle.net/10449/21745
File in questo prodotto:
File Dimensione Formato  
2013 MBE Hunt et al.pdf

non disponibili

Licenza: Tutti i diritti riservati (All rights reserved)
Dimensione 342.97 kB
Formato Adobe PDF
342.97 kB Adobe PDF   Visualizza/Apri   Richiedi una copia

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10449/21745
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 36
  • ???jsp.display-item.citation.isi??? 37
social impact