We describe a new method for the sex determination of tissue originating from Oryctolagus cuniculus (European rabbit), Lepus europaeus (European brown hare) and Lepus timidus (mountain hare) based on PCR-RFLP analysis of point mutations that differentiate the ZFX and ZFY gene sequences. Among several applications, this PCR-RFLP method could be used to investigate gender ratio and evaluate the population dynamics of these species using samples collected when sex cannot be identified.

Fontanesi, L.; Tazzoli, M.; Pecchioli, E.; Hauffe, H.C.; Robinson, T.J.; Russo, V. (2008). Sexing European rabbits (Oryctolagus cuniculus), European brown hares (Lepus europaeus) and mountain hares (Lepus timidus) with ZFX and ZFY loci. MOLECULAR ECOLOGY RESOURCES, 8 (6): 1294-1296. doi: 10.1111/j.1755-0998.2008.02167.x handle: http://hdl.handle.net/10449/20475

Sexing European rabbits (Oryctolagus cuniculus), European brown hares (Lepus europaeus) and mountain hares (Lepus timidus) with ZFX and ZFY loci

Pecchioli, Elena;Hauffe, Heidi Christine;
2008-01-01

Abstract

We describe a new method for the sex determination of tissue originating from Oryctolagus cuniculus (European rabbit), Lepus europaeus (European brown hare) and Lepus timidus (mountain hare) based on PCR-RFLP analysis of point mutations that differentiate the ZFX and ZFY gene sequences. Among several applications, this PCR-RFLP method could be used to investigate gender ratio and evaluate the population dynamics of these species using samples collected when sex cannot be identified.
Hare
Leporidae
Molecular sexing
Rabbit
ZFX
ZFY
2008
Fontanesi, L.; Tazzoli, M.; Pecchioli, E.; Hauffe, H.C.; Robinson, T.J.; Russo, V. (2008). Sexing European rabbits (Oryctolagus cuniculus), European brown hares (Lepus europaeus) and mountain hares (Lepus timidus) with ZFX and ZFY loci. MOLECULAR ECOLOGY RESOURCES, 8 (6): 1294-1296. doi: 10.1111/j.1755-0998.2008.02167.x handle: http://hdl.handle.net/10449/20475
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