Knowledge about the sequence-based genetic diversity of a crop species is important in order to develop highly informative genotyping assays, which will eventually positively impact breeding practice. Diversity data were obtained from two pools of 185 and 75 accessions each, representing most of the species belonging to the genus Malus, by re-sequencing 27 gene-specific amplicons and by screening 237 Malus × domestica SNPs using the multiplex genotyping technology SNPlex™. Nucleotide diversity and insertion/deletion rates in M. × domestica were estimated as π = 0.0037 and 1/333 bp, respectively. The SNP frequency was estimated as 0.0194 (1 SNP/52 bp) while within a single apple cultivar an average of one SNP in every 455 bp was found. We also investigated transferability (T SNP) of the heterozygous state of SNPs across the species M. × domestica and the genus Malus. Raw re-sequencing showed that 12–15% of M. × domestica SNPs are transferable to a second M. × domestica cultivar, however T SNP rose to ~41% with SNPs selected for high minor allele frequency. T SNP of chosen SNPs averaged ~27% in the two M. × domestica-related species, Malus sieversii and Malus sylvestris, but was much lower in more distantly related species. On the basis of T SNP, simulations, and empirical results, we calculated that a close-design, multiplexed genotyping array with at least 2,000 SNPs is required for building a highly saturated linkage maps within any M. × domestica cross. The same array would gradually lose informativeness in increasingly phylogenetically distant Malus species.

Micheletti, D.; Troggio, M.; Zharkikh, A.; Costa, F.; Malnoy, M.A.; Velasco, R.; Salvi, S. (2011). Genetic diversity of the genus Malus and implications for linkage mapping with SNPs. TREE GENETICS & GENOMES, 7 (4): 857-868. doi: 10.1007/s11295-011-0380-8 handle: http://hdl.handle.net/10449/20025

Genetic diversity of the genus Malus and implications for linkage mapping with SNPs

Micheletti, Davide;Troggio, Michela;Costa, Fabrizio;Malnoy, Mickael Arnaud;Velasco, Riccardo;Salvi, Silvio
2011-01-01

Abstract

Knowledge about the sequence-based genetic diversity of a crop species is important in order to develop highly informative genotyping assays, which will eventually positively impact breeding practice. Diversity data were obtained from two pools of 185 and 75 accessions each, representing most of the species belonging to the genus Malus, by re-sequencing 27 gene-specific amplicons and by screening 237 Malus × domestica SNPs using the multiplex genotyping technology SNPlex™. Nucleotide diversity and insertion/deletion rates in M. × domestica were estimated as π = 0.0037 and 1/333 bp, respectively. The SNP frequency was estimated as 0.0194 (1 SNP/52 bp) while within a single apple cultivar an average of one SNP in every 455 bp was found. We also investigated transferability (T SNP) of the heterozygous state of SNPs across the species M. × domestica and the genus Malus. Raw re-sequencing showed that 12–15% of M. × domestica SNPs are transferable to a second M. × domestica cultivar, however T SNP rose to ~41% with SNPs selected for high minor allele frequency. T SNP of chosen SNPs averaged ~27% in the two M. × domestica-related species, Malus sieversii and Malus sylvestris, but was much lower in more distantly related species. On the basis of T SNP, simulations, and empirical results, we calculated that a close-design, multiplexed genotyping array with at least 2,000 SNPs is required for building a highly saturated linkage maps within any M. × domestica cross. The same array would gradually lose informativeness in increasingly phylogenetically distant Malus species.
Apple
High-throughput genotyping
SNP
Settore AGR/07 - GENETICA AGRARIA
2011
Micheletti, D.; Troggio, M.; Zharkikh, A.; Costa, F.; Malnoy, M.A.; Velasco, R.; Salvi, S. (2011). Genetic diversity of the genus Malus and implications for linkage mapping with SNPs. TREE GENETICS & GENOMES, 7 (4): 857-868. doi: 10.1007/s11295-011-0380-8 handle: http://hdl.handle.net/10449/20025
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10449/20025
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