Two field exercises were carried out to compare chlorophyll a fluorescence measurements taken in the field by field teams working on the same project. In the first exercise (2007, Passo Pura, Ampezzo, Udine, Northern Italy) the operators took measurements on the same leaf areas (maintaining fixed leaf clips); in the second (2009, Monterotondo Marittimo, Grosseto, Central Italy) the teams worked independently, but addressing a common research question. The results of the first exercise showed that: (a) FV/FM was stable and had little variation among teams and instruments; (b) the results from the different teams correlated well; (c) the most suitable parameters of fast kinetics analysis are those measured on the normalized fluorescence transients. In the second exercise, when the teams worked independently, the results were much more variable and the correlations between measurements of different operators were weak. These results suggest that field chlorophyll a fluorescence measurements taken by different teams/operators can be comparable only if particular care is taken to the internal variability of the samples and a standardized sampling strategy is applied. A statistically sound representation of a population can be then reached.

Bussotti, F.; Pollastrini, M.; Cascio, C.; Desotgiu, R.; Gerosa, G.; Marzuoli, R.; Nali, C.; Lorenzini, G.; Pellegrini, E.; Carucci, M.G.; Salvatori, E.; Fusaro, L.; Piccotto, M.; Malaspina, P.; Manfredi, A.; Roccotello, E.; Toscano, S.; Gottardini, E.; Cristofori, A.; Fini, A.; Weber, D.; Baldassarre, V.; Barbanti, L.; Monti, A.; Strasser, R.J. (2011). Conclusive remarks. Reliability and comparability of chlorophyll fluorescence data from several field teams. ENVIRONMENTAL AND EXPERIMENTAL BOTANY, 73 (1): 116-119. doi: 10.1016/j.envexpbot.2010.10.023 handle: http://hdl.handle.net/10449/20014

Conclusive remarks. Reliability and comparability of chlorophyll fluorescence data from several field teams

Gottardini, Elena;Cristofori, Antonella;
2011-01-01

Abstract

Two field exercises were carried out to compare chlorophyll a fluorescence measurements taken in the field by field teams working on the same project. In the first exercise (2007, Passo Pura, Ampezzo, Udine, Northern Italy) the operators took measurements on the same leaf areas (maintaining fixed leaf clips); in the second (2009, Monterotondo Marittimo, Grosseto, Central Italy) the teams worked independently, but addressing a common research question. The results of the first exercise showed that: (a) FV/FM was stable and had little variation among teams and instruments; (b) the results from the different teams correlated well; (c) the most suitable parameters of fast kinetics analysis are those measured on the normalized fluorescence transients. In the second exercise, when the teams worked independently, the results were much more variable and the correlations between measurements of different operators were weak. These results suggest that field chlorophyll a fluorescence measurements taken by different teams/operators can be comparable only if particular care is taken to the internal variability of the samples and a standardized sampling strategy is applied. A statistically sound representation of a population can be then reached.
Chlorophyll fluorescence
Field exercises
FV/FM
Harmonization
Intercalibration
Fluorescenza della clorofilla
Esercizio in campo
Fv/Fm
Armonizzazione
Intercalibrazione
Settore BIO/01 - BOTANICA GENERALE
2011
Bussotti, F.; Pollastrini, M.; Cascio, C.; Desotgiu, R.; Gerosa, G.; Marzuoli, R.; Nali, C.; Lorenzini, G.; Pellegrini, E.; Carucci, M.G.; Salvatori, E.; Fusaro, L.; Piccotto, M.; Malaspina, P.; Manfredi, A.; Roccotello, E.; Toscano, S.; Gottardini, E.; Cristofori, A.; Fini, A.; Weber, D.; Baldassarre, V.; Barbanti, L.; Monti, A.; Strasser, R.J. (2011). Conclusive remarks. Reliability and comparability of chlorophyll fluorescence data from several field teams. ENVIRONMENTAL AND EXPERIMENTAL BOTANY, 73 (1): 116-119. doi: 10.1016/j.envexpbot.2010.10.023 handle: http://hdl.handle.net/10449/20014
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