Alpine pastures are a complex, multidimensional terrestrial habitat in which animal and plant species interact with each other and with large numbers of bacteria and fungi (microbiota), forming an ecological network that extends both above ground and within the soil. Although soil-, plant-, and animal-associated microbiota are known to provide fundamental services for their hosts, microbial diversity of ecosystems in general, and Alpine pastures in particular, is still largely unexplored. In addition, the extent to which climate changes in Europe and especially in the Alpine space will impact such micro-biodiversity is completely unknown. Here, we investigated changes in bacterial and fungal diversity associated with more than 900 samples of soil, rhi- zosphere (Carex spp. and Festuca spp.), invertebrates (nematodes, collembolans, earthworms, beetles) and vertebrates (using faecal eDNA of hares, wild ungulates and livestock) along an elevational gradient spanning 1,500 metres (a proxy for climate change). Characterization of bacterial and fungal communities of each sample was performed by targeting the bacterial 16S rRNA gene V3-V4 region and the fungal ITS2 loci as barcodes for amplicon sequencing-based meta-taxonomics. Diversity metrics of ASVs (amplicon sequence variants) and phylogenetic distances (Bray-Curtis, Unifrac) were used in redundancy analyses (db-RDA) to compare microbiota composition across sample types and elevation gradient. We found that soil, plant, invertebrate, and vertebrate microbiota were characterized by only partially overlapping bacterial and fungal communities, displaying specific associations with host, elevation, temperature, nutrient availability, and plant community composition. Fae- cal microbiota of wild and farmed animals revealed different patterns across bacterial and fungal microbiota, with bacterial communities being markedly shaped by host-interaction dynamics, and fungal communities showing closer associations with habitat and elevation. Analyses of fungal and bacterial taxa shared between sample types established greater overlaps between soil, rhizosphere and soil-dwelling invertebrates, compared to other invertebrates and vertebrates, highlighting above/ belowground and host/habitat-specific associations in the Alpine meta-community.

Galla, G.; Praeg, N.; Rzehak, T.; Scholz, M.; Colla, F.; Seeber, J.; Illmer, P.; Hauffe, H.C. (2024). Bacterial and fungal diversity of Alpine pastures. In: ForumAlpinum 2024: The Alps – a Refuge of Bio- and Geodiversity!?, Kranjska Gora, Slovenia, 13-14 June 2024. Ljubljana: 26. handle: https://hdl.handle.net/10449/88656

Bacterial and fungal diversity of Alpine pastures

Galla, G.
;
Scholz, M.;Hauffe, H. C.
2024-01-01

Abstract

Alpine pastures are a complex, multidimensional terrestrial habitat in which animal and plant species interact with each other and with large numbers of bacteria and fungi (microbiota), forming an ecological network that extends both above ground and within the soil. Although soil-, plant-, and animal-associated microbiota are known to provide fundamental services for their hosts, microbial diversity of ecosystems in general, and Alpine pastures in particular, is still largely unexplored. In addition, the extent to which climate changes in Europe and especially in the Alpine space will impact such micro-biodiversity is completely unknown. Here, we investigated changes in bacterial and fungal diversity associated with more than 900 samples of soil, rhi- zosphere (Carex spp. and Festuca spp.), invertebrates (nematodes, collembolans, earthworms, beetles) and vertebrates (using faecal eDNA of hares, wild ungulates and livestock) along an elevational gradient spanning 1,500 metres (a proxy for climate change). Characterization of bacterial and fungal communities of each sample was performed by targeting the bacterial 16S rRNA gene V3-V4 region and the fungal ITS2 loci as barcodes for amplicon sequencing-based meta-taxonomics. Diversity metrics of ASVs (amplicon sequence variants) and phylogenetic distances (Bray-Curtis, Unifrac) were used in redundancy analyses (db-RDA) to compare microbiota composition across sample types and elevation gradient. We found that soil, plant, invertebrate, and vertebrate microbiota were characterized by only partially overlapping bacterial and fungal communities, displaying specific associations with host, elevation, temperature, nutrient availability, and plant community composition. Fae- cal microbiota of wild and farmed animals revealed different patterns across bacterial and fungal microbiota, with bacterial communities being markedly shaped by host-interaction dynamics, and fungal communities showing closer associations with habitat and elevation. Analyses of fungal and bacterial taxa shared between sample types established greater overlaps between soil, rhizosphere and soil-dwelling invertebrates, compared to other invertebrates and vertebrates, highlighting above/ belowground and host/habitat-specific associations in the Alpine meta-community.
Microbiota
16S rRNA gene sequencing
ITS2
Metataxonomy
Pasture
2024
Galla, G.; Praeg, N.; Rzehak, T.; Scholz, M.; Colla, F.; Seeber, J.; Illmer, P.; Hauffe, H.C. (2024). Bacterial and fungal diversity of Alpine pastures. In: ForumAlpinum 2024: The Alps – a Refuge of Bio- and Geodiversity!?, Kranjska Gora, Slovenia, 13-14 June 2024. Ljubljana: 26. handle: https://hdl.handle.net/10449/88656
File in questo prodotto:
File Dimensione Formato  
2024 Galla 26.pdf

accesso aperto

Tipologia: Versione editoriale (Publisher’s layout)
Licenza: Tutti i diritti riservati (All rights reserved)
Dimensione 43.59 kB
Formato Adobe PDF
43.59 kB Adobe PDF Visualizza/Apri

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10449/88656
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus ND
  • ???jsp.display-item.citation.isi??? ND
social impact