A comparative profiling of the secondary metabolism of cyanobacteria isolated from Italian perialpine lakes has been attempted. Cultures of Aphanizomenon flos-aquae, Dolichospermum lemmermannii, Microcystis aeruginosa, Planktothrix rubescens, and Tychonema bourrellyi were obtained from lakes Garda, Idro, and Caldonazzo. LC-MS/MS analysis were used for characterizing the strains’ extracts. A target analyis aimed at assessing the the toxic profile (microcystins, nodularins, anatoxins, cylindrospermopsins, PSP) was performed; it revealed the production of toxic peptides (microcystins) in M. aeruginosa and P. rubescens, of toxic alkaloids (anatoxin-a and possibly some paralytic shellfish toxins) in T. Bourrellyi, and finally no production of toxins in Aph. flos-aquae and D. Lemmermannii. An untargeted analysis aimed at comparing the capability of the cyanobacterial species to produce peptidic metabolites, allowed the detection of over 300 different peptides, in a mass range between 400 and 2000 Da. The majority of compounds with masses between 500 and 1200 Da (corresponding to many non-ribosomal peptides) was produced by the two species M. aeruginosa and P. rubescens, which show a much higher capability of producing these compounds compared to the others. Microcystins, aeruginosins and anabaenopeptins were the most represented classes of compounds.

Cerasino, L.; Salmaso, N. (2018). Metabolic profiles of cyanobacteria isolated from Italian perialpine lakes. In: The 18th internatonal conference on harmful algae (ICHA2018): from ecosystems to socio-ecosystems, Nantes, France, 21-26 October 2018. Nantes: 183. url: https://www.icha2018.com/medias/content/files/ICHA_2018_Abstract_Book_v7.pdf handle: http://hdl.handle.net/10449/51164

Metabolic profiles of cyanobacteria isolated from Italian perialpine lakes

Cerasino, L.
Primo
;
Salmaso, N.
Ultimo
2018-01-01

Abstract

A comparative profiling of the secondary metabolism of cyanobacteria isolated from Italian perialpine lakes has been attempted. Cultures of Aphanizomenon flos-aquae, Dolichospermum lemmermannii, Microcystis aeruginosa, Planktothrix rubescens, and Tychonema bourrellyi were obtained from lakes Garda, Idro, and Caldonazzo. LC-MS/MS analysis were used for characterizing the strains’ extracts. A target analyis aimed at assessing the the toxic profile (microcystins, nodularins, anatoxins, cylindrospermopsins, PSP) was performed; it revealed the production of toxic peptides (microcystins) in M. aeruginosa and P. rubescens, of toxic alkaloids (anatoxin-a and possibly some paralytic shellfish toxins) in T. Bourrellyi, and finally no production of toxins in Aph. flos-aquae and D. Lemmermannii. An untargeted analysis aimed at comparing the capability of the cyanobacterial species to produce peptidic metabolites, allowed the detection of over 300 different peptides, in a mass range between 400 and 2000 Da. The majority of compounds with masses between 500 and 1200 Da (corresponding to many non-ribosomal peptides) was produced by the two species M. aeruginosa and P. rubescens, which show a much higher capability of producing these compounds compared to the others. Microcystins, aeruginosins and anabaenopeptins were the most represented classes of compounds.
Metabolomics
Cyanobacteria
Perialpine lakes
LC-MS
2018
Cerasino, L.; Salmaso, N. (2018). Metabolic profiles of cyanobacteria isolated from Italian perialpine lakes. In: The 18th internatonal conference on harmful algae (ICHA2018): from ecosystems to socio-ecosystems, Nantes, France, 21-26 October 2018. Nantes: 183. url: https://www.icha2018.com/medias/content/files/ICHA_2018_Abstract_Book_v7.pdf handle: http://hdl.handle.net/10449/51164
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