Anthocyanins are considered to be beneficial and may protect from chronic inflammation. Thus, investigations on anthocyanin-rich extracts and single constituents are of great interest. We investigated a blackberry extract, containing a spectrum of anthocyanins, in comparison to two sub-fractions comprising cyanidin-3-(6''-dioxalylglucoside) and cyanidin-3-(malonylglucoside), respectively, regarding their impact on inflammation-related cytokine expression and secretion in human macrophages. Relative gene transcription and cytokine secretion of IL-6 and TNF-alpha were measured using qRT-PCR and cytokine bead-based immunoassay, respectively. The THP-1 monocyte cells served as a cell model to mimic the inflammatory response. Therefor cells were differentiated with phorbol-12-myristate-13-acetate into macrophages which were pre-incubated with physiologically relevant concentrations of blackberry extract or single compounds. Subsequent stimulation with lipopolysaccharides from E.coli induced gene transcription and cytokine release. Cyanidin-3-(malonylglucoside) had no impact on LPS-induced IL6 and TNF-alpha expression. In contrast, the blackberry extract and cyanidin-3-(6''-dioxalylglucoside) caused further stimulatory effects for the transcription and secretion of IL-6 at higher concentrations. The secretion of TNF-alpha was increased as well by both, yet with no impact on the transcript level after 3 h of incubation. The results clearly show that single as well as combinatory effects might be of relevance for the immune-stimulatory impact of the blackberry extract.

Cenk, E.; Pahlke, G.; Oertel, A.; Martens, S.; Matros, A.; Mock, H.; Marko, D. (2016). Impact of a blackberry extract and single anthocyanins on the inflammatory response of human macrophage THP-1 cells. In: 10th World Congress on Polyphenols Applications, Porto, Portugal, June 29-July 1, 2016. doi: 10.18143/JISANH_v3i4_1377 handle: http://hdl.handle.net/10449/34384

Impact of a blackberry extract and single anthocyanins on the inflammatory response of human macrophage THP-1 cells

Martens, Stefan;
2016

Abstract

Anthocyanins are considered to be beneficial and may protect from chronic inflammation. Thus, investigations on anthocyanin-rich extracts and single constituents are of great interest. We investigated a blackberry extract, containing a spectrum of anthocyanins, in comparison to two sub-fractions comprising cyanidin-3-(6''-dioxalylglucoside) and cyanidin-3-(malonylglucoside), respectively, regarding their impact on inflammation-related cytokine expression and secretion in human macrophages. Relative gene transcription and cytokine secretion of IL-6 and TNF-alpha were measured using qRT-PCR and cytokine bead-based immunoassay, respectively. The THP-1 monocyte cells served as a cell model to mimic the inflammatory response. Therefor cells were differentiated with phorbol-12-myristate-13-acetate into macrophages which were pre-incubated with physiologically relevant concentrations of blackberry extract or single compounds. Subsequent stimulation with lipopolysaccharides from E.coli induced gene transcription and cytokine release. Cyanidin-3-(malonylglucoside) had no impact on LPS-induced IL6 and TNF-alpha expression. In contrast, the blackberry extract and cyanidin-3-(6''-dioxalylglucoside) caused further stimulatory effects for the transcription and secretion of IL-6 at higher concentrations. The secretion of TNF-alpha was increased as well by both, yet with no impact on the transcript level after 3 h of incubation. The results clearly show that single as well as combinatory effects might be of relevance for the immune-stimulatory impact of the blackberry extract.
Anthocyanins
Inflammation
Cenk, E.; Pahlke, G.; Oertel, A.; Martens, S.; Matros, A.; Mock, H.; Marko, D. (2016). Impact of a blackberry extract and single anthocyanins on the inflammatory response of human macrophage THP-1 cells. In: 10th World Congress on Polyphenols Applications, Porto, Portugal, June 29-July 1, 2016. doi: 10.18143/JISANH_v3i4_1377 handle: http://hdl.handle.net/10449/34384
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