Erysiphe necator is the causal agent of powdery mildew (PM), one of the most destructive diseases of grapevine. Powdery mildew is controlled by sulphur-based and synthetic fungicides, which every year are dispersed in the environment. This is why PM resistant varieties should become a priority for sustainable grapevine and wine production. Resistance can be achieved by knocking-out susceptibility S-genes, such as those residing at genetic loci known as MLO (Mildew Locus O). All MLO S-genes of dicots belong to the phylogenetic clade V, including grapevine genes VvMLO7, 11, 13, which are up-regulated during PM infection, and VvMLO6, which is not up-regulated. Before adopting a gene editing approach to knockout candidate S-genes, the evidence that loss-of-function of MLO genes can reduce PM susceptibility is necessary. We show that the knock-down through RNA interference of VvMLO6, 7, 11 and 13. Knock-down of VvMLO6, 11 and 13, alone or combined, did not decrease PM severity, whereas the knock-down of VvMLO7in combination with VvMLO6 and VvMLO11, reduces severity up to 77%. Cell wall appositions (papillae), a response to PM attack, were present in both resistant and susceptible lines, but were larger in resistant lines

Pessina, S.; Lenzi, L.; Perazzolli, M.; Dalla Costa, L.; Campa, M.; Velasco, R.; Malnoy, M.A. (2016). MLO genes silencing reduces susceptibility to powdery mildew in grapevine. In: X International symposium on grapevine physiology and biotechnology, Verona, June 13-16, 2016: 66. handle: http://hdl.handle.net/10449/33797

MLO genes silencing reduces susceptibility to powdery mildew in grapevine

Pessina, Stefano;Lenzi, Luisa;Perazzolli, Michele;Dalla Costa, Lorenza;Campa, Manuela;Velasco, Riccardo;Malnoy, Mickael Arnaud
2016-01-01

Abstract

Erysiphe necator is the causal agent of powdery mildew (PM), one of the most destructive diseases of grapevine. Powdery mildew is controlled by sulphur-based and synthetic fungicides, which every year are dispersed in the environment. This is why PM resistant varieties should become a priority for sustainable grapevine and wine production. Resistance can be achieved by knocking-out susceptibility S-genes, such as those residing at genetic loci known as MLO (Mildew Locus O). All MLO S-genes of dicots belong to the phylogenetic clade V, including grapevine genes VvMLO7, 11, 13, which are up-regulated during PM infection, and VvMLO6, which is not up-regulated. Before adopting a gene editing approach to knockout candidate S-genes, the evidence that loss-of-function of MLO genes can reduce PM susceptibility is necessary. We show that the knock-down through RNA interference of VvMLO6, 7, 11 and 13. Knock-down of VvMLO6, 11 and 13, alone or combined, did not decrease PM severity, whereas the knock-down of VvMLO7in combination with VvMLO6 and VvMLO11, reduces severity up to 77%. Cell wall appositions (papillae), a response to PM attack, were present in both resistant and susceptible lines, but were larger in resistant lines
2016
Pessina, S.; Lenzi, L.; Perazzolli, M.; Dalla Costa, L.; Campa, M.; Velasco, R.; Malnoy, M.A. (2016). MLO genes silencing reduces susceptibility to powdery mildew in grapevine. In: X International symposium on grapevine physiology and biotechnology, Verona, June 13-16, 2016: 66. handle: http://hdl.handle.net/10449/33797
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