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ItalianoErysiphe necator is the causal agent of powdery mildew (PM), one of the most destructive diseases of grapevine. Powdery mildew is controlled by sulphur-based and synthetic fungicides, which every year are dispersed in the environment. This is why PM resistant varieties should become a priority for sustainable grapevine and wine production. Resistance can be achieved by knocking-out susceptibility S-genes, such as those residing at genetic loci known as MLO (Mildew Locus O). All MLO S-genes of dicots belong to the phylogenetic clade V, including grapevine genes VvMLO7, 11, 13, which are up-regulated during PM infection, and VvMLO6, which is not up-regulated. Before adopting a gene editing approach to knockout candidate S-genes, the evidence that loss-of-function of MLO genes can reduce PM susceptibility is necessary. We show that the knock-down through RNA interference of VvMLO6, 7, 11 and 13. Knock-down of VvMLO6, 11 and 13, alone or combined, did not decrease PM severity, whereas the knock-down of VvMLO7in combination with VvMLO6 and VvMLO11, reduces severity up to 77%. Cell wall appositions (papillae), a response to PM attack, were present in both resistant and susceptible lines, but were larger in resistant lines
| Citation: | Pessina , S.; Lenzi, L.; Perazzolli, M.; Dalla Costa, L.; Campa, M.; Velasco, R.; Malnoy, M. (2016). MLO genes silencing reduces susceptibility to powdery mildew in grapevine. In: X International symposium on grapevine physiology and biotechnology, Verona, June 13-16, 2016: 66. handle: http://hdl.handle.net/10449/33797 |
| Internal authors: | |
| Organization unit: | Department of Genomics and Biology of Fruit Crops # CRI Department of Sustainable Agro-Ecosystems and Bioresources # CRI |
| Authors: | Pessina , S.; Lenzi, L.; Perazzolli, M.; Dalla Costa, L.; Campa, M.; Velasco, R.; Malnoy, M. |
| Title: | MLO genes silencing reduces susceptibility to powdery mildew in grapevine |
| Issue Date: | 2016 |
| Scientific Disciplinary Area: | Settore BIO/11 - Biologia Molecolare |
| Language: | English |
| Nature of content: | Abstract in Atti di convegno/Conference abstract |
| Appears in Collections: | 03 - Conference object |
Files in This Item:
| File | Description | Type | License | |
|---|---|---|---|---|
| 2016 VR 66.pdf | N/A | Tutti i diritti riservati (All rights reserved) | Open AccessView/Open |
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