The endosymbiotic bacterium Wolbachia is considered as a potential biological tool for controlling invasive insect pest populations such as Drosophila suzukii (Dsuz), which has recently invaded European countries and come out as an emerging threat for fresh fruit cultivation. Recent reports have shown that European Dsuz harbors a new Wolbachia strain named wSuzi, related to the strong CI-causing wRi strain of D. simulans. Nothing is known behind this novel host-microbial integrative biology as yet. Thus, to get more insights into this symbiotic relationship, we have examined Wolbachia tissue tropism in adult Dsuz individuals sampled according to different age status. Quantitative Real Time PCR (qRT-PCR) was used to calculate density of Wolbachia in reproductive as well as in different somatic tissues of 1, 7, 14 and 21 days old Dsuz flies of both sexes. We detected significant differences in Wolbachia titre 1) between reproductive organs of males and females, 2) among different somatic tissues, 3) based on different age groups. Notably, in male gonads, Wolbachia density substantially decreases with age, suggesting the possible use of Wolbachia-rich young males to evaluate their CI-inducing capability. Results will be further discussed with those obtained from D. simulans and D. melanogaster harboring their respective wRi and wMel strains. Thereby this approach will help in evaluating the potential use of wSuzi to control Dsuz population in future

Kaur, R.; Hummel, T.; Rota Stabelli, O.; Miller, W.J. (2014). What can we infer from symbionts titre in their respective Drosophila host tissues?. In: 8th International Wolbachia Conference, Igls, Innsbruck, Austria, 6-11 June 2014: 49. url: http://wolbachia2014.org/ handle: http://hdl.handle.net/10449/24097

What can we infer from symbionts titre in their respective Drosophila host tissues?

Rota Stabelli, Omar;
2014-01-01

Abstract

The endosymbiotic bacterium Wolbachia is considered as a potential biological tool for controlling invasive insect pest populations such as Drosophila suzukii (Dsuz), which has recently invaded European countries and come out as an emerging threat for fresh fruit cultivation. Recent reports have shown that European Dsuz harbors a new Wolbachia strain named wSuzi, related to the strong CI-causing wRi strain of D. simulans. Nothing is known behind this novel host-microbial integrative biology as yet. Thus, to get more insights into this symbiotic relationship, we have examined Wolbachia tissue tropism in adult Dsuz individuals sampled according to different age status. Quantitative Real Time PCR (qRT-PCR) was used to calculate density of Wolbachia in reproductive as well as in different somatic tissues of 1, 7, 14 and 21 days old Dsuz flies of both sexes. We detected significant differences in Wolbachia titre 1) between reproductive organs of males and females, 2) among different somatic tissues, 3) based on different age groups. Notably, in male gonads, Wolbachia density substantially decreases with age, suggesting the possible use of Wolbachia-rich young males to evaluate their CI-inducing capability. Results will be further discussed with those obtained from D. simulans and D. melanogaster harboring their respective wRi and wMel strains. Thereby this approach will help in evaluating the potential use of wSuzi to control Dsuz population in future
Wolbachia titre
2014
Kaur, R.; Hummel, T.; Rota Stabelli, O.; Miller, W.J. (2014). What can we infer from symbionts titre in their respective Drosophila host tissues?. In: 8th International Wolbachia Conference, Igls, Innsbruck, Austria, 6-11 June 2014: 49. url: http://wolbachia2014.org/ handle: http://hdl.handle.net/10449/24097
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10449/24097
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